Enhanced H2 production by deletion of the Tfx family DNA-binding protein in the hyperthermophilic archaeon Thermococcus onnurineus NA1 SCIE SCOPUS

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Title
Enhanced H2 production by deletion of the Tfx family DNA-binding protein in the hyperthermophilic archaeon Thermococcus onnurineus NA1
Author(s)
Lee, Seong Hyuk; Lee, Sung Mok; Kang, Sung Gyun; Lee, Hyun Sook
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)
Alternative Author(s)
이성혁; 이성목; 강성균; 이현숙
Publication Year
2021-10-14
Abstract
A gene encoding an archaeal transcription regulator (TON_1525) homologous to the Tfx family DNA-binding protein has been identified to affect gene expression of carbon monoxide metabolism in the hyperthermophilic archaeon Thermococcus onnurineus NA1. To broadly understand gene regulation by the TON_1525 gene, a gene deletion mutant (Δ1525) was constructed and transcriptome changes were investigated. A total of 351 genes were found to be differentially expressed in the Δ1525 mutant. The expression levels of the hydrogenase genes were significantly changed; mbh (membrane-bound hydrogenase) and mch (membrane-bound carbon monoxide-dependent hydrogenase) genes were increased, and mfh2 (membrane-bound formate-dependent hydrogenase) and soluble hydrogenase genes were decreased. The Δ1525 mutant was cultured in a medium supplemented with maltodextrin or carbon monoxide to investigate whether changes in gene expression lead to physiological changes. The maximum H2 production rates of the Δ1525 mutant were significantly enhanced on both substrates compared to the wild-type strain. These results indicate that the TON_1525 gene can affect the expression of various genes including mbh and mch, consequently regulating the metabolism of T. onnurineus NA1. This study expands our understanding of the functional role of the Tfx family DNA-binding proteins. © 2021
ISSN
0360-3199
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/42115
DOI
10.1016/j.ijhydene.2021.08.075
Bibliographic Citation
International Journal of Hydrogen Energy, v.46, no.71, pp.35189 - 35197, 2021
Publisher
Elsevier Ltd
Keywords
H2 production; Hydrogenase; Tfx family DNA-Binding protein; Thermococcus onnurineus NA1
Type
Article
Language
English
Document Type
Article
Publisher
Elsevier Ltd
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