Sediment quality assessment combining chemical and biological (non) target analysis SCIE SCOPUS

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Title
Sediment quality assessment combining chemical and biological (non) target analysis
Author(s)
Choi, Youmi; Kim, Moonkoo; Ha, Sung Yong; Han, Gi Myung; Yim, Un Hyuk; Jung, Jee Hyun
KIOST Author(s)
Choi, You Mi(최유미)Kim, Moon Koo(김문구)Ha, Sung Yong(하성용)Han, Gi Myung(한기명)Yim, Un Hyuk(임운혁)Jung, Jee Hyun(정지현)
Alternative Author(s)
최유미; 김문구; 하성용; 한기명; 임운혁; 정지현
Publication Year
2021-09
Abstract
Aquatic sediments act as a storage for diverse mixtures of organic and inorganic contaminants. Nevertheless, most evaluations of contaminated sediments have been limited to the assessment of concentrations of target compounds and lethal effects on some test species. To identify the organic contaminants causing sub-lethal effects of contaminated sediment, this study combined chemical and biological (non)target analysis involving comprehensive two-dimensional gas chromatography coupled with a time-of-flight Mass Spectrometer (GCxGC/ToF-MS) analysis, embryonic malformation and high-throughput sequencing (RNA-seq) analysis on developing flounder. Polycyclic aromatic hydrocarbons were more abundant in the sediment extract of Yeongil Bay (SEY), while Jinhae Bay (SEJ) was contaminated with a large amount of unidentified chemicals. The unidentified chemicals of SEJ included branched alkanes, oxygenated cycloalkanes, heterogeneous hydrocarbons, and other unknown compounds. Percentage of pericardial edema was the highest in embryonic flounder exposed to SEY. Consistent with the morphogenesis results, the expression level of genes related to heart formation including the nkx2.5 and robo1 was greater in embryonic flounder exposed to SEY. In the analyses of differential gene expression profiles (cutoff P < 0.05), by RNA-seq, embryos exposed to SEJ showed changes related to cell differentiation, cell part morphogenesis, neurogenesis, and neuron development. Genes related to neurogenesis and positive regulation of molecular functions variated significantly in embryos exposed to SEY. These results demonstrated the advantages of combining target and non-target analysis to accurately evaluate the major chemical groups causing sediment toxicity. Therefore, this work provided a useful approach to tracking and revealing the causes of toxic effects and identifying potential toxic mechanisms.
ISSN
0166-445X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/41529
DOI
10.1016/j.aquatox.2021.105883
Bibliographic Citation
AQUATIC TOXICOLOGY, v.238, 2021
Publisher
Elsevier BV
Subject
UNRESOLVED COMPLEX MIXTURE; MASAN BAY; DEVELOPMENTAL TOXICITY; AROMATIC-HYDROCARBONS; CONTACT ASSAY; FISH EMBRYOS; DANIO-RERIO; CRUDE-OIL; CONTAMINATION; APOPTOSIS
Keywords
Sediment; Toxicity; NGS; GCxGC; ToF-MS; Non-target screening
Type
Article
Language
English
Document Type
Article
Publisher
Elsevier BV
Related Researcher
Research Interests

Oil Spill Environmental Forensics,Persistent Organic Pollutants,Marine Aerosol,유류오염 환경법과학,지속성유기오염물질,해양 에어로졸

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