Changes in gene expression profile of medaka with acute toxicity of Arochlor 1260, a polychlorinated biphenyl mixture SCIE SCOPUS

Cited 21 time in WEB OF SCIENCE Cited 27 time in Scopus
Title
Changes in gene expression profile of medaka with acute toxicity of Arochlor 1260, a polychlorinated biphenyl mixture
Author(s)
Yum, Seungshic; Woo, Seonock; Kagami, Yoshihiro; Park, Hong-Seog; Ryu, Jae-Chun
KIOST Author(s)
Yum, Seung Shic(염승식)
Publication Year
2010-01
Abstract
Differential gene expression profiling was performed with a cDNA microarray in the liver tissue of the medaka fish, Oryzias latipes, after exposure to Arochlor 1260, a polychlorinated biphenyl (PCB) mixture, which is used as a coolant and insulating fluid for transformers and capacitors and is classified as a persistent organic pollutant. Twenty-six differentially expressed candidate genes were identified. The expression of 12 genes was up-regulated and that of 14 genes was down-regulated. These genes are associated with the cytoskeleton, development, endocrine/reproduction, immunity, metabolism, nucleic acid/protein binding, and signal transduction, or are uncategorized. The transcription of molecular biomarkers known to be involved in endocrine disruption (e.g., vitellogenins, choriogenins, and estrogen receptor alpha) was highly upregulated. The same tendencies in gene expression changes were observed with real-time quantitative PCR (qRT-PCR) analysis, which was conducted to examine 12 selected candidate genes. These genes could be used as molecular biomarkers for biological responses to toxic chemicals, especially endocrine disrupting and carcinogenic chemical contamination in aquatic environments. (C) 2009 Elsevier Inc. All rights reserved.
ISSN
1532-0456
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4150
DOI
10.1016/j.cbpc.2009.08.007
Bibliographic Citation
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v.151, no.1, pp.51 - 56, 2010
Publisher
ELSEVIER SCIENCE INC
Subject
ARYL-HYDROCARBON RECEPTOR; SQUAMOUS-CELL CARCINOMA; EGG ENVELOPE CHORION; ORYZIAS-LATIPES; ESTROGEN-RECEPTOR; JAPANESE MEDAKA; MOLECULAR-CLONING; COMPLEMENT COMPONENTS; MICROARRAY ANALYSIS; KERATIN EXPRESSION
Keywords
Oryzias latipes; Arochlor 1260; Polychlorinated biphenyls (PCBs); Differential gene expression profile; Real-time quantitative PCR; cDNA microarray
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE INC
Related Researcher
Research Interests

Marine Genomics,Environmental Genomics,Novel biomaterials,해양생물유전체,생태환경유전체,바이오신소재

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