First characterisation of the populations and immune-related activities of hemocytes from two edible gastropod species, the disk abalone, Haliotis discus discus and the spiny top shell, Turbo cornutus SCIE SCOPUS

Cited 63 time in WEB OF SCIENCE Cited 64 time in Scopus
Title
First characterisation of the populations and immune-related activities of hemocytes from two edible gastropod species, the disk abalone, Haliotis discus discus and the spiny top shell, Turbo cornutus
Author(s)
Donaghy, Ludovic; Hong, Hyun-Ki; Lambert, Christophe; Park, Heung-Sik; Shim, Won Joon; Choi, Kwang-Sik
KIOST Author(s)
Park, Heung Sik(박흥식)Shim, Won Joon(심원준)
Publication Year
2010-01
Abstract
The disk abalone Haliotis discus discus and the spiny top shell Turbo cornutus are edible gastropod species of high economic value, mainly in Asia. Mortality outbreaks and variations in worldwide stock abundance have been reported and suggested to be associated, at least in part, with pathogenic infections. Ecology, biology and immunology of both species are currently not well documented. The characterisation of the immune systems of these species is necessary to further assess the responses of H. discus discus and T cornutus to environmental, chemical and disease stresses. In the present study, we investigated the morphology and immune-related activities of hemocytes in both species using light microscopy and flow cytometry. Two types of hemocytes were identified in the disk abalone hemolymph, blast-like cells and hyalinocytes; whereas four main hemocyte types were distinguished in the spiny top shell, blast-like cells, type I and II hyalinocytes, and granulocytes. Flow cytometric analysis also revealed differences between cell types in immune-related activities. Three subsets of hemocytes, defined by differing lysosomal characteristics, were observed in the hemolymph of the spiny top shell, and only one in the disk abalone. Phagocytic activity was higher in H. discus discus hemocytes than in T cornutus hemocytes, and the kinetics of PMA-stimulated oxidative activity was different between hemocytes of the disk abalone and the spiny top shell. Finally our results suggest for the first time a predominant mitochondrial origin of oxidative activity in gastropod hemocytes. (C) 2009 Elsevier Ltd. All rights reserved.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4148
DOI
10.1016/j.fsi.2009.10.006
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.28, no.1, pp.87 - 97, 2010
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
SNAIL BIOMPHALARIA-GLABRATA; FLOW-CYTOMETRIC ANALYSIS; EUROPEAN FLAT OYSTER; LYMNAEA-STAGNALIS GASTROPODA; NITRIC-OXIDE PRODUCTION; CRASSOSTREA-GIGAS; REACTIVE OXYGEN; DEFENSE-MECHANISMS; RESPIRATORY BURST; EASTERN OYSTERS
Keywords
Disk abalone; Spiny top shell; Hemocytes; Flow cytometry; Phagocytosis; Oxidative activity; Lysosomes
Type
Article
Language
English
Document Type
Review
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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