Development and Evaluation of Olive Flounder cyp1a1-Luciferase Assay for Effective Detection of CYP1A-Inducing Contaminants in Coastal Sediments SCIE SCOPUS

Cited 1 time in WEB OF SCIENCE Cited 2 time in Scopus
Title
Development and Evaluation of Olive Flounder cyp1a1-Luciferase Assay for Effective Detection of CYP1A-Inducing Contaminants in Coastal Sediments
Author(s)
Jung, Jee Hyun; Yim, Bora; Jeong, Sol; Yoon, Mee-Sup; Kim, Bo-Mi; Ha, Sung Yong; Kim, Moonkoo; Rhee, Jae-Sung; Lee, Young-Mi
KIOST Author(s)
Jung, Jee Hyun(정지현)Ha, Sung Yong(하성용)Kim, Moon Koo(김문구)
Alternative Author(s)
정지현; 하성용; 김문구
Publication Year
2020-12-01
Abstract
Flounders have been widely used as indicator species for monitoring the benthic environment of marine coastal regions owing to their habitat and feeding preferences in or on sandy sediments. Here, a single-step, sensitive, specific, and simple luciferase assay was developed, using the olive flounder cyp1a1 gene, for effective detection of CYP1A-inducing contaminants in coastal sediments. The developed cyp1a1-luciferase assay was highly sensitive to the widely used CYP1A inducers 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), benzo[a]pyrene (B[a]P), and 3,3',4,4',5-pentachlorobiphenyl (PCB 126). In the case of TCDD, significant dose-dependent increases in luciferase activity (0.3-300 ng/L) were detected. The assay was more sensitive to PCB 126 than to B[a]P. The assay also involved the highly sensitive expression of luciferase to extracted mixtures of PCBs and polycyclic aromatic hydrocarbons (PAHs) collected from coastal sediments. PCBs were more capable of cyp1a1 induction in the assay system at small doses than PAHs in environmental samples. Using the cyp1a1-luciferase assay along with water or sediment chemistry will certainly aid in diagnosing CYP1A-inducing contaminants in coastal environments.
ISSN
0013-936X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/41381
DOI
10.1021/acs.est.0c06921
Bibliographic Citation
ENVIRONMENTAL SCIENCE & TECHNOLOGY, v.54, no.23, pp.15170 - 15179, 2020
Publisher
AMER CHEMICAL SOC
Subject
POLYCYCLIC AROMATIC-HYDROCARBONS; DIOXIN-LIKE ACTIVITY; IN-VIVO ASSAY; ANTIOXIDANT PARAMETERS; CYTOCHROME-P450 GENES; TRANSGENIC ZEBRAFISH; MOLECULAR RESPONSES; ORGANIC-COMPOUNDS; MASAN BAY; PCB 126
Type
Article
Language
English
Document Type
Article
Publisher
AMER CHEMICAL SOC
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