Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress SCIE SCOPUS

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Title
Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress
Author(s)
Lee, Hyo-Geun; Kim, Hyun-Soo; Oh, Jae-Young; Lee, Dae-Sung; Yang, Hye-Won; Kang, Min-Cheol; Kim, Eun A; Kang, Nalae; Kim, Junseong; Heo, Soo Jin; Jeon, You-Jin
KIOST Author(s)
Kim, Eun A(김은아)Kang, Nalae(강나래)Kim, Junseong(김준성)Heo, Soo Jin(허수진)
Alternative Author(s)
김은아; 강나래; 김준성; 허수진
Publication Year
2021-01
Abstract
A comprehensive antioxidant evaluation was performed on enzymatic hydrolysates of Stichopus japonicus (S. japonicus) using Vero cells and zebrafish models for in vitro and in vivo studies, respectively. S. japonicus was hydrolyzed with food-grade enzymes (alcalase, alpha-chymotrypsin, flavourzyme, kojizyme, neutrase, papain, pepsin, protamex, and trypsin), and the free radical scavenging activities were screened via electron spin resonance (ESR) spectroscopy. According to the results, the enzymatic hydrolysates contained high protein and relatively low polysaccharide and sulfate contents. Among these hydrolysates, the alpha-chymotrypsin assisted hydrolysate from S. japonicus (alpha-chy) showed high yield and protein content, and strong hydroxyl radical scavenging activity. Therefore, alpha-chy was chosen for further purification. The alpha-chy was fractionated by ultrafiltration into three ultrafiltration (UF) fractions based on their molecular weight: >10 kDa (alpha-chy-I), 5-10 kDa (alpha-chy-II), and <5 kDa (alpha-chy-III), and we evaluated their antioxidant properties in H2O2 exposed Vero cells. The alpha-chy and its UF fractions significantly decreased the intracellular reactive oxygen species (ROS) generation and increased cell viability in H2O2 exposed Vero cells. Among them, alpha-chy-III effectively declined the intracellular ROS levels and increased cell viability and exhibited protection against H2O2 induced apoptotic damage. Furthermore, alpha-chy-III remarkably attenuated the cell death, intracellular ROS and lipid peroxidation in H2O2 exposed zebrafish embryos. Altogether, our findings demonstrated that alpha-chy and its alpha-chy-III from S. japonicus possess strong antioxidant activities that could be utilized as a bioactive ingredient for functional food industries.
ISSN
2076-3921
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/41367
DOI
10.3390/antiox10010110
Bibliographic Citation
ANTIOXIDANTS, v.10, no.1, pp.1 - 15, 2021
Publisher
MDPI
Keywords
antioxidant; red sea cucumber; Stichopus japonicus; enzyme-assisted extract; oxidative stress
Type
Article
Language
English
Document Type
Article
Publisher
MDPI
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

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