Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress
SCIE
SCOPUS
Cited 17 time in
WEB OF SCIENCE
Cited 17 time in
Scopus
-
Title
- Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress
-
Author(s)
- Lee, Hyo-Geun; Kim, Hyun-Soo; Oh, Jae-Young; Lee, Dae-Sung; Yang, Hye-Won; Kang, Min-Cheol; Kim, Eun A; Kang, Nalae; Kim, Junseong; Heo, Soo Jin; Jeon, You-Jin
- KIOST Author(s)
- Kim, Eun A(김은아); Kang, Na Lae(강나래); Kim, Jun Seong(김준성); Heo, Soo Jin(허수진)
-
Alternative Author(s)
- 김은아; 강나래; 김준성; 허수진
-
Publication Year
- 2021-01
-
Abstract
- A comprehensive antioxidant evaluation was performed on enzymatic hydrolysates of Stichopus japonicus (S. japonicus) using Vero cells and zebrafish models for in vitro and in vivo studies, respectively. S. japonicus was hydrolyzed with food-grade enzymes (alcalase, alpha-chymotrypsin, flavourzyme, kojizyme, neutrase, papain, pepsin, protamex, and trypsin), and the free radical scavenging activities were screened via electron spin resonance (ESR) spectroscopy. According to the results, the enzymatic hydrolysates contained high protein and relatively low polysaccharide and sulfate contents. Among these hydrolysates, the alpha-chymotrypsin assisted hydrolysate from S. japonicus (alpha-chy) showed high yield and protein content, and strong hydroxyl radical scavenging activity. Therefore, alpha-chy was chosen for further purification. The alpha-chy was fractionated by ultrafiltration into three ultrafiltration (UF) fractions based on their molecular weight: >10 kDa (alpha-chy-I), 5-10 kDa (alpha-chy-II), and <5 kDa (alpha-chy-III), and we evaluated their antioxidant properties in H2O2 exposed Vero cells. The alpha-chy and its UF fractions significantly decreased the intracellular reactive oxygen species (ROS) generation and increased cell viability in H2O2 exposed Vero cells. Among them, alpha-chy-III effectively declined the intracellular ROS levels and increased cell viability and exhibited protection against H2O2 induced apoptotic damage. Furthermore, alpha-chy-III remarkably attenuated the cell death, intracellular ROS and lipid peroxidation in H2O2 exposed zebrafish embryos. Altogether, our findings demonstrated that alpha-chy and its alpha-chy-III from S. japonicus possess strong antioxidant activities that could be utilized as a bioactive ingredient for functional food industries.
-
ISSN
- 2076-3921
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/41367
-
DOI
- 10.3390/antiox10010110
-
Bibliographic Citation
- ANTIOXIDANTS, v.10, no.1, pp.1 - 15, 2021
-
Publisher
- MDPI
-
Keywords
- antioxidant; red sea cucumber; Stichopus japonicus; enzyme-assisted extract; oxidative stress
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.