alpha-Poly-l-lysine functions as an adipogenic inducer in 3T3-L1 preadipocytes SCIE SCOPUS

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Title
alpha-Poly-l-lysine functions as an adipogenic inducer in 3T3-L1 preadipocytes
Author(s)
Lee, Kyeong Won; An, Young Jun; Lee, Janet; Lee, Jung-Hyun; Yim, Hyung-Soon
KIOST Author(s)
Lee, Kyeong Won(이경원)Young, Jun An(안영준)Lee, Janet(이자넷)Lee, Jung Hyun(이정현)Yim, Hyung Soon(임형순)
Alternative Author(s)
이경원; 안영준; 이자넷; 이정현; 임형순
Publication Year
2021-04
Abstract
alpha-Poly-l-lysine (PLL) has been used for various purposes such as cell attachment, immunization, and molecular delivery, and is known to be cytotoxic to several cell lines. Here, we studied the effect of PLL on the adipogenesis of 3T3-L1 cells and investigated the underlying mechanism. Differentiation media containing PLL with a molecular weight (MW) greater than 4 kDa enhanced lipid droplet formation and increased adipogenic marker levels, indicating an increase in adipocyte differentiation. PLL with a molecular weight between 30 and 70 kDa was more effective than PLL of other sizes in 3T3-L1 cell differentiation. Moreover, PLL induced 3T3-L1 adipogenesis in insulin-free adipocyte differentiation medium. Incubation with insulin and PLL exhibited greater adipogenesis than insulin treatment only even at a high concentration. PLL stimulated insulin signaling and augmented the signaling pathway when it was added with insulin. While PLL did not activate the glucocorticoid receptor, which is phosphorylated by dexamethasone (DEX), it showed a positive effect on the cAMP signal pathway when preadipocytes were treated with PLL and 3-isobutyl-1-methylxanthine (IBMX). Consistent with these results, incubation with PLL and DEX without IBMX induced adipocyte differentiation. We also observed that the mitotic clonal expansion phase was the critical stage in adipogenesis for inducing the effects of PLL. These results suggest that PLL functions as an adipogenic inducer in 3T3-L1 preadipocytes and PLL has a direct effect on insulin signaling, one of the main regulatory pathways.
ISSN
0939-4451
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/41326
DOI
10.1007/s00726-020-02932-2
Bibliographic Citation
AMINO ACIDS, v.53, no.4, pp.587 - 596, 2021
Publisher
SPRINGER WIEN
Keywords
α -Poly-l-lysine; Adipogenesis; Insulin signaling
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER WIEN
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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