Amplicon pyrosequencing 을 사용한 ddp 유전체 다양성

Abstract

Dimethylsulfoniopropionate(DMSP) is a sulfur compound produced by phytoplankton. After released into seawater by trophic interactions such as viral lysis and grazing, DMSP is rapidly degraded by diverse enzymes via two major metabolic pathways. While the majority of DMSP could be transferred to microbial biomass by enzymatic cleavage as bacterial sulfer and carbon sources, a smaller fraction could be used to produce dimethylsulfide (DMS) which is a major source of biogenic sulfur to the atmosphere. The oxidation products of DMS in the atmosphere form cloud condensation nuclei and ultimately influence albedo. In this study, sequences of dddP gene which is involved in DMS production was investigated by using a barcoded amplicon pyrosequencing method to elucidate diversity of bacteria participating in DMS production in the NW Pacific Ocean.After denoising, trimming and removing of PCR errors, total of 78,641 sequences were obtained from 48 samples. These sequences could be classified into 119 OTUs by employing a criterion of 97% sequence similarity. Interestingly, single OTU was found to be most dominant (average of 32%) genotype in most stations except a station located on continental shelf area of the East China Sea, whereas other dominant OTUs showed apparent spatial distribution patterns. OTUs closely related with Roseobacter clades which are important in DMSP demethylation pathway was athways. While the majority of DMSP could be transferred to microbial biomass by enzymatic cleavage as bacterial sulfer and carbon sources, a smaller fraction could be used to produce dimethylsulfide (DMS) which is a major source of biogenic sulfur to the atmosphere. The oxidation products of DMS in the atmosphere form cloud condensation nuclei and ultimately influence albedo. In this study, sequences of dddP gene which is involved in DMS production was investigated by using a barcoded amplicon pyrosequencing method to elucidate diversity of