DNA바코드를 활용, 여러종을 포함한 네트 시료에서의 종 탐색

Abstract

Evaluation of species occurrence and distribution is a first step toward biodiversity assessment and is a necessary procedure in the studies of biogeography, conservation biology and ecology. For marine organisms, however it is often difficult to identify some species in a small size as well as the eggs and larvae whose morphological characters are not well recognizable. Here we present a molecular approach based on the DNA barcode to detect presence of a target species in the multi-species mixed samples.We developed species-specific primers and probes for 17 marine organisms common in the Korean waters which can amplify mitochondrial DNA sequence in the 5region of the COI gene and distinguish the presence of each species in the netted samples. Through the realtime-PCR with the primer and probe sets, we were able to examine the presence of the target species in the 330 um net samples collected from Ioedo and Tongyoung waters.Enabling species identification from the environmental samples, this molecular technique using the DNA barcode opens a new perspective for the study of marine organisms as well as the assessment of marine biodiversity.fficult to identify some species in a small size as well as the eggs and larvae whose morphological characters are not well recognizable. Here we present a molecular approach based on the DNA barcode to detect presence of a target species in the multi-species mixed samples.We developed species-specific primers and probes for 17 marine organisms common in the Korean waters which can amplify mitochondrial DNA sequence in the 5region of the COI gene and distinguish the presence of each species in the netted samples. Through the realtime-PCR with the primer and probe sets, we were able to examine the presence of the target species in the 330 um net samples collected from Ioedo and Tongyoung waters.Enabling species identification from the environmental samples, this molecular technique using t