DNA바코드를 활용한 현장 시료에서의 참돔 알 판별

Abstract

Pagrus major, the red seabream, is an important fishery species in East Asia including China, Japan and Korea in both capture and culture fisheries. In recent years, the global warming results in changes of the marine environment as well as the marine ecosystem around the Korean peninsula. Therefore, identification of fish eggs becomes more important for detection of spawning periods and grounds in establishment of their proper management and conservation plans. However, the eggs of most species in Korean waters except Engraulis japonicus and Maurolicus muelleri are difficult in identification. The morphological characters of the eggs that are used in species identification are sometimes similar and even overlap among the species. In this study, we developed the species-specific probes and primers from the mitochondrial DNA COI sequence so called the DNA barcode which enabled us to identify the eggs of the red seabream in the net samples. Using the probes and primers, we verified the presence of red seabream eggs in multi-species mixed samples via the process of realtime-PCR which was indicated by the result of next generation sequencing of the samples.l as the marine ecosystem around the Korean peninsula. Therefore, identification of fish eggs becomes more important for detection of spawning periods and grounds in establishment of their proper management and conservation plans. However, the eggs of most species in Korean waters except Engraulis japonicus and Maurolicus muelleri are difficult in identification. The morphological characters of the eggs that are used in species identification are sometimes similar and even overlap among the species. In this study, we developed the species-specific probes and primers from the mitochondrial DNA COI sequence so called the DNA barcode which enabled us to identify the eggs of the red seabream in the net samples. Using the probes and primers, we verified the presence of re