Effects of pCO2 on the hatching of Artemia franciscana cysts(A new CO2 equilibrator based on airlift pump)

Abstract

Marine organisms during early life history are susceptible to increased carbon dioxide. To study the responses of marine organisms on the elevated pCO2, we investigated the hatching rate of Artemia franciscana cysts through a new system. pCO2 treatments in seawater (25 L) were 87 ± 5 ~ 663 ± 1 ppm (10 batches, 29.5 ± 0.03 ℃, 24.2 psu, pH 8.08 ± 0.01 ~ 8.69 ± 0.03) and 503 ± 1 ~ 1,324 ± 2 ppm (8 batches, 29.6 ± 0.02 ℃, 24.2 psu, pH 7.70 ± 0.01 ~ 8.13 ± 0.01). CO2 extracted from a new equilibrator based on air pump was measured by NDIR (Non-dispersive infrared gas sensor). Under the each pCO2 conditions, Artemia franciscana cysts (11/ml) were incubated for 24 hours. The linear regression between pCO2 and the hatching rates was -0.021x+91.261(R2=0.2375, p<0.05). Therefore, the increased pCO2 would decrease the hatching rate of Artemia franciscana cysts.CO2 treatments in seawater (25 L) were 87 ± 5 ~ 663 ± 1 ppm (10 batches, 29.5 ± 0.03 ℃, 24.2 psu, pH 8.08 ± 0.01 ~ 8.69 ± 0.03) and 503 ± 1 ~ 1,324 ± 2 ppm (8 batches, 29.6 ± 0.02 ℃, 24.2 psu, pH 7.70 ± 0.01 ~ 8.13 ± 0.01). CO2 extracted from a new equilibrator based on air pump was measured by NDIR (Non-dispersive infrared gas sensor). Under the each pCO2 conditions, Artemia franciscana cysts (11/ml) were incubated for 24 hours. The linear regression between pCO2 and the hatching rates was -0.021x+91.261(R2=0.2375, p<0.05). Therefore, the increased pCO2 would decrease the hatching rate of Artemia franciscana cysts.