인도네시아 술라웨시 해역의 동물플랑크톤 집단의 유전적 분석

Abstract

The Indonesian archipelago comprises about 18,110 islands with coastlines totaling 108,920km. About 78% of the Indonesian territory is covered by coastal waters which comprise the epicenter of the world marine biodiversity (Mulyadi, 2012). The high biodiversity is well reflected in the pelagic ecosystem. In the present study, we analyzed the species composition of zooplankton samples collected from the EWIN 2014 international cruise survey carried out in the Sulawesi sea during November 30 to December 16, 2014. The plankton samples were collected with a NORPAC net (mesh size: 0.33mm). A part of the mitochondrial COI gene, 313 bp in length, called DNA barcode was sequenced with the samples to identify the comprising species by massive sequencing of the DNA barcode through Next Generation Sequencing (Illumina MiSeq). Approximately 168 molecular operation taxonomic units MOTUs with similarity higher than 85% to the known species were obtained from the samples. Arthropods are the most abundant organism group in the community (76% of the MOTUs) and other phyla such as Chordata (10%), Mollusca (5%), Cinidaria(4%), Chaetognatha (2%), Echinodermata, Sipuncula, Annelida, Placozoa (each <1%) are included. The most abundant species in terms of the DNA reads in each animal group are Subeucalanus mucronatus (Arthropoda), Bolinichthys sp. (Chordata), Creseis chierchiae (Mollusca), Abylopsis eschscholtzi (Cnidaria), and AidanosagitThe high biodiversity is well reflected in the pelagic ecosystem. In the present study, we analyzed the species composition of zooplankton samples collected from the EWIN 2014 international cruise survey carried out in the Sulawesi sea during November 30 to December 16, 2014. The plankton samples were collected with a NORPAC net (mesh size: 0.33mm). A part of the mitochondrial COI gene, 313 bp in length, called DNA barcode was sequenced with the samples to identify the comprising species by massive sequencing of the DNA barcode through Next Generation Sequencing (Illumina MiSeq). Approximately 168 molecular operation taxonomic units MOTUs with similarity higher than 85% to the known species were obtained from the samples. Arthropods are the most abundant organism group in the community (76% of the MOTUs) and other phyla such as Chordata (10%), Mollusca (5%), Cinidaria(4%), Chaetognatha (2%), Echinodermata, Sipuncula, Annelida, Placozoa (each <1%) are included. The most abundant species in terms of the DNA reads in each animal group are Subeucalanus mucronatus (Arthropoda), Bolinichthys sp. (Chordata), Creseis chierchiae (Mollusca), Abylopsis eschscholtzi (Cnidaria), and Aidanosagit