Development of a rapid and effective method for preparing delicate dinoflagellates for scanning electron microscopy SCIE SCOPUS

Cited 23 time in WEB OF SCIENCE Cited 25 time in Scopus
Title
Development of a rapid and effective method for preparing delicate dinoflagellates for scanning electron microscopy
Author(s)
Jung, Seung Won; Joo, Hyoung Min; Park, Joon Sang; Lee, Jin Hwan
KIOST Author(s)
Jung, Seung Won(정승원)
Publication Year
2010-06
Abstract
We developed a rapid and effective procedure for scanning electron microscopy of three delicate dinoflagellates, Karlodinium micrum, Akashiwo sanguinea, and Heterocapsa niei. Good results were obtained when the specimens were fixed with a modified Parducz's fixative (2% osmium tetroxide:saturated mercuric chloride = 5:1 v/v) for 10 min, washed in 0.05 M sodium cacodylate trihydrate buffer for 2 min, dehydrated in tert-butanol for 10 min and dried with hexamethyldisilazane in air for 3 min in a fume hood because reagents are very toxic. This method could be completed in 25 min. Compared with other preparative techniques, the present protocol has significant advantages for SEM observation by limiting distortion of delicate specimens and reducing the preparation time.
ISSN
0921-8971
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4085
DOI
10.1007/s10811-009-9461-6
Bibliographic Citation
JOURNAL OF APPLIED PHYCOLOGY, v.22, no.3, pp.313 - 317, 2010
Publisher
SPRINGER
Subject
BIOLOGICAL SPECIMENS; SP-NOV; SEM; HEXAMETHYLDISILAZANE; TISSUES
Keywords
Delicate dinoflagellates; Hexamethyldisilazane; Parducz fixative; Sodium cacodylate buffer; tert-Butanol
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

Phytoplatnkon taxonomy,Phytoplatnkon physiology,Phycosphere,식물플랑크톤 분류,식물플랑크톤 생리,식물플랑크톤, 바이러스, 박테리아 관계

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