Development of a rapid and effective method for preparing delicate dinoflagellates for scanning electron microscopy
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Title
- Development of a rapid and effective method for preparing delicate dinoflagellates for scanning electron microscopy
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Author(s)
- Jung, Seung Won; Joo, Hyoung Min; Park, Joon Sang; Lee, Jin Hwan
- KIOST Author(s)
- Jung, Seung Won(정승원)
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Alternative Author(s)
- 정승원
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Publication Year
- 2010-06
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Abstract
- We developed a rapid and effective procedure for scanning electron microscopy of three delicate dinoflagellates, Karlodinium micrum, Akashiwo sanguinea, and Heterocapsa niei. Good results were obtained when the specimens were fixed with a modified Parducz's fixative (2% osmium tetroxide:saturated mercuric chloride = 5:1 v/v) for 10 min, washed in 0.05 M sodium cacodylate trihydrate buffer for 2 min, dehydrated in tert-butanol for 10 min and dried with hexamethyldisilazane in air for 3 min in a fume hood because reagents are very toxic. This method could be completed in 25 min. Compared with other preparative techniques, the present protocol has significant advantages for SEM observation by limiting distortion of delicate specimens and reducing the preparation time.
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ISSN
- 0921-8971
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4085
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DOI
- 10.1007/s10811-009-9461-6
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Bibliographic Citation
- JOURNAL OF APPLIED PHYCOLOGY, v.22, no.3, pp.313 - 317, 2010
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Publisher
- SPRINGER
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Subject
- BIOLOGICAL SPECIMENS; SP-NOV; SEM; HEXAMETHYLDISILAZANE; TISSUES
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Keywords
- Delicate dinoflagellates; Hexamethyldisilazane; Parducz fixative; Sodium cacodylate buffer; tert-Butanol
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Type
- Article
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Language
- English
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Document Type
- Article
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