Evaluation of anti-inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages SCIE SCOPUS

Cited 176 time in WEB OF SCIENCE Cited 200 time in Scopus
Title
Evaluation of anti-inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages
Author(s)
Heo, Soo-Jin; Yoon, Weon-Jong; Kim, Kil-Nam; Ahn, Gin-Nae; Kang, Sung-Myung; Kang, Do-Hyung; Affan, Abu; Oh, Chulhong; Jung, Won-Kyo; Jeon, You-Jin
KIOST Author(s)
Heo, Soo Jin(허수진)Kang, Do-Hyung(강도형)Oh, Chulhong(오철홍)
Publication Year
2010-08
Abstract
In this study, potential anti-inflammatory effect of fucoxanthin isolated from brown algae was assessed via inhibitory effect of nitric oxide (NO) production in lipopolysaccharide (LPS) induced RAW 264.7 macrophage cells. The Myagropsis myagroides was selected for further experiments due to its profound NO inhibitory effect, and was partitioned with different organic solvents. Highest NO inhibitory effect was detected in the chloroform fraction, and the active compound was identified as fucoxanthin, a kind of carotenoid available in brown algae evidenced high correlation with the inhibitory effect of NO production (r(2) = 0.9511). Though, fucoxanthin significantly inhibited the NO production, it slightly reduced the prostaglandin E-2 (PGE(2)) production. The inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) protein expressions were inhibited by fucoxanthin. Further, RT-PCR analysis indicated that the iNOS and COX-2 mRNA expressions were suppressed by fucoxanthin. Moreover, the release of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-6 (IL-6), and the mRNA expression levels of those cytokines were reduced by the addition of fucoxanthin in a dose-dependent manner. Hence, these results suggest that the use of fucoxanthin may be a useful therapeutic approach for the various inflammatory diseases. (C) 2010 Elsevier Ltd. All rights reserved.
ISSN
0278-6915
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4053
DOI
10.1016/j.fct.2010.05.003
Bibliographic Citation
FOOD AND CHEMICAL TOXICOLOGY, v.48, no.8-9, pp.2045 - 2051, 2010
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
Fucoxanthin; Myagropsis myagroides; Brown algae; Anti-inflammation; Macrophage; Lipopolysaccharide
Type
Article
Language
English
Document Type
Article
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Related Researcher
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse