Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase
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Title
- Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase
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Author(s)
- Ekanayake, Prashani Mudika; De Zoysa, Mahanama; Kang, Hyun-Sil; Wan, Qiang; Jee, Youngheun; Lee, Youn-Ho; Kim, Sang-Jin; Lee, Jehee
- KIOST Author(s)
- Lee, Youn Ho(이윤호)
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Alternative Author(s)
- 이윤호; 김상진
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Publication Year
- 2008-03
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Abstract
- Catalase is an antioxidant enzyme that plays a significant role in protection against oxidative stress by detoxification of hydrogen peroxide (H2O2). A gene coding for a putative catalase was isolated from the disk abalone (Haliotis discus discus) cDNA library and denoted as Ab-catalase. The full-length (2864 bp) Ab-catatase cDNA contained 1,503 bp open reading frame (ORF), encoding 501 amino acid residues with 56 kDa predicted molecular weight. The deduced amino acid sequence of Ab-catalase has characteristic features of catalase family such as catalytic site motif ((61)FNRERIPERVVHAKGAG(77)), heme-ligand signature motif ((RLYSYSDT158)-R-351), NADPH and heme binding residues. Phylogenetic and pairwise identity results indicated that Ab-catatase is more similar to scallop (Chlamys farreri) catalase with 80% amino acid identity except for other reported disk abalone catalase sequences. Constitutive Ab-catalase expression was detected in gill, mantle, gonad, hemocytes, abductor muscle and digestive tract in tissue specific manner. Ab-catatase mRNA was up-regulated in gill and digestive tract tissues for the first 3 h post injection of H2O2, showing the inducible ability of abalone catalase against oxidative stress generated by H2O2. The purified recombinant catatase showed 30,000 U/mg enzymatic activity against H2O2 and biochemical properties of higher thermal stability and broad spectrum of pH. Our results suggest that abalone catatase may play an important role in regulating oxidative stress by scavenging H2O2. (C) 2007 Elsevier Ltd. All rights reserved.
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ISSN
- 1050-4648
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/40477
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DOI
- 10.1016/j.fsi.2007.11.007
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Bibliographic Citation
- FISH & SHELLFISH IMMUNOLOGY, v.24, no.3, pp.267 - 278, 2008
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Publisher
- ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
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Subject
- BEEF-LIVER CATALASE; ANTIOXIDANT ENZYMES; HYDROGEN-PEROXIDE; MYTILUS-GALLOPROVINCIALIS; MARINE-INVERTEBRATES; SEQUENCE ALIGNMENT; MOLECULAR-CLONING; NADPH BINDING; RESPONSES; STRESS
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Keywords
- abalone; antioxidant; catalase; cDNA; H2O2; reactive oxygen species; tissue expression
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Type
- Article
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Language
- English
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Document Type
- Article
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