유세포 분석기와 Neutral Red Retention Assay를 이용한 자연산 참굴(Crassostrea gigas)의 면역력 측정 SCOPUS KCI

Title
유세포 분석기와 Neutral Red Retention Assay를 이용한 자연산 참굴(Crassostrea gigas)의 면역력 측정
Alternative Title
Assessment of immune parameters of the wild pacific Oyster (Crassostrea gigas) using a flow cytometry and neutral red retention assay
Author(s)
홍현기; 강현실; 김영옥; 최광식
KIOST Author(s)
Kim, Young Ok(김영옥)
Alternative Author(s)
김영옥
Publication Year
2012
Abstract
Hemocyte parameters of the wild Pacific oyster Crassostrea gigas inhabiting intertidal zones in small bays (Gwangyang and Jinhae Bay) on the southern coast of Korea were evaluated using flow cytometry and neutral red retention (NRR) assay. Morphological features, cell count, mortality, DNA damage, phagocytosis, and lysosomal membrane stability of hemocytes were analyzed. Three types of hemocytes were identified in the oyster hemolymph: granulocytes, hyalinocytes, and blast-like cells. Immune related functions of hemocyte including phagocytosis and lysosomal membrane stability were significantly different among the study areas (P<0.05), while cell count, mortality, and DNA damage of hemocytes were not significantly different. In Gwangyang Bay, phagocytosis of granulocytes and lysosomal membrane stability of oyster hemocytes inhabiting inside bay were significantly lower than those of oyster hemocytes in outside bay (P<0.05), indicating that oysters in inside bay of Gwangyang were relatively suppressed the immunological function in hemocytes. Contrary to Gwangyang Bay, immune parameters of oyster hemocytes in Jinhae Bay not showed the difference between sampling sites. In conclusion, flow cytometry and NRR assay using oyster hemocyte has a powerful tool to investigate the cell level in a short time due to no-preprocessing of material.
ISSN
1598-141X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40441
DOI
10.4217/OPR.2012.34.2.137
Bibliographic Citation
Ocean and Polar Research, v.34, no.2, pp.137 - 149, 2012
Keywords
Crassostrea gigas; flow cytometry; neutral red retention assay; hemocyte; DNA damage; phagocytosis; lysosomal membrane stability
Type
Article
Language
Korean
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse