Molecular and histological identification of Marteilioides infection in Suminoe Oyster Crassostrea ariakensis, Manila Clam Ruditapes philippinarum and Pacific Oyster Crassostrea gigas on the south coast of Korea SCIE SCOPUS

Cited 7 time in WEB OF SCIENCE Cited 8 time in Scopus
Title
Molecular and histological identification of Marteilioides infection in Suminoe Oyster Crassostrea ariakensis, Manila Clam Ruditapes philippinarum and Pacific Oyster Crassostrea gigas on the south coast of Korea
Author(s)
Yanin, Limpanont; Kang, Hyun-Sil; Hong, Hyun-Ki; Jeung, Hee-Do; Kim, Bong-Kyu; Thanh Cuong Le; Kim, Young-Ok; Choi, Kwang-Sik
KIOST Author(s)
Kim, Young Ok(김영옥)
Publication Year
2013-11
Abstract
The oyster ovarian parasite Marteilioides chungmuensis has been reported from Korea and Japan, damaging the oyster industries. Recently, Marteilioides-like organisms have been identified in other commercially important marine bivalves. In this study, we surveyed Marteilioides infection in the Manila clam Ruditapes philippinarum, Suminoe oyster Crassostrea ariakensis, and Pacific oyster Crassostrea gigas, using histology and Marteilioides-specific small subunit (SSU) rDNA PCR. The SSU rDNA sequence of M. chungmuensis (1716 bp) isolated from C gigas in Tongyoung bay was 99.9% similar to that of M. chungmuensis reported in japan. Inclusions of multi-nucleated bodies in the oocytes, typical of Marteilioides infection, were identified for the first time in Suminoe oysters. The SSU rDNA sequence of a Marteilioides-like organism isolated from Suminoe oysters was 99.9% similar to that of M. chungmuensis. Marteilioides sp. was also observed from 7 Manila clams of 1840 individuals examined, and the DNA sequences of which were 98.2% similar to the known sequence of M. chungmuensis. Unlike Marteilioides infection of Pacific oysters, no remarkable pathological symptoms, such as large multiple lumps on the mantle, were observed in infected Suminoe oysters or Manila clams. Distribution of the infected Manila clams, Suminoe oysters and Pacific oysters was limited to small bays on the south coast, suggesting that the southern coast is the enzootic area of Marteilioides infection. (C) 2013 Elsevier Inc. All rights reserved.
ISSN
0022-2011
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40423
DOI
10.1016/j.jip.2013.08.008
Bibliographic Citation
JOURNAL OF INVERTEBRATE PATHOLOGY, v.114, no.3, pp.277 - 284, 2013
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Subject
OVARIAN PARASITE; CHUNGMUENSIS PARAMYXEA; PROTOZOAN PARASITE; DNA PROBES
Keywords
Marteilioides chungmuensis; Crassostrea gigas; Ruditapes philippinarum; Crassostrea ariakensis; SSU rDNA; Nested PCR
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
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