Single cell genome analysis of an uncultured heterotrophic stramenopile
SCIE
SCOPUS
Cited 39 time in
WEB OF SCIENCE
Cited 42 time in
Scopus
-
Title
- Single cell genome analysis of an uncultured heterotrophic stramenopile
-
Author(s)
- Roy, Rajat S.; Price, Dana C.; Schliep, Alexander; Cai, Guohong; Korobeynikov, Anton; Yoon, Hwan Su; Yang, Eun Chan; Bhattacharya, Debashish
- KIOST Author(s)
- Yang, Eun Chan(양은찬)
-
Alternative Author(s)
- 양은찬
-
Publication Year
- 2014-04-24
-
Abstract
- A broad swath of eukaryotic microbial biodiversity cannot be cultivated in the lab and is therefore inaccessible to conventional genome-wide comparative methods. One promising approach to study these lineages is single cell genomics (SCG), whereby an individual cell is captured from nature and genome data are produced from the amplified total DNA. Here we tested the efficacy of SCG to generate a draft genome assembly from a single sample, in this case a cell belonging to the broadly distributed MAST-4 uncultured marine stramenopiles. Using de novo gene prediction, we identified 6,996 protein-encoding genes in the MAST-4 genome. This genetic inventory was sufficient to place the cell within the ToL using multigene phylogenetics and provided preliminary insights into the complex evolutionary history of horizontal gene transfer (HGT) in the MAST-4 lineage.
-
ISSN
- 2045-2322
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/40416
-
DOI
- 10.1038/srep04780
-
Bibliographic Citation
- SCIENTIFIC REPORTS, v.4, 2014
-
Publisher
- NATURE PUBLISHING GROUP
-
Subject
- HORIZONTAL GENE-TRANSFER; EVOLUTIONARY HISTORY; ANALYSIS SUPPORTS; ALGAL GENES; AMPLIFICATION; PATTERNS; DIVERSIFICATION; CRYPTOPHYTES; HAPTOPHYTES; METAGENOME
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.