DNA Barcoding of Metazoan Zooplankton Copepods from South Korea SCIE SCOPUS

Cited 21 time in WEB OF SCIENCE Cited 26 time in Scopus
Title
DNA Barcoding of Metazoan Zooplankton Copepods from South Korea
Author(s)
Baek, Su Youn; Jang, Kuem Hee; Choi, Eun Hwa; Ryu, Shi Hyun; Kim, Sang Ki; Lee, Jin Hee; Lim, Young Jin; Lee, Jimin; Jun, Jumin; Kwak, Myounghai; Lee, Young-Sup; Hwang, Jae-Sam; Maran, Balu Alagar Venmathi; Chang, Cheon Young; Kim, Il-Hoi; Hwang, Ui Wook
KIOST Author(s)
Lee, Ji Min(이지민)
Alternative Author(s)
이지민
Publication Year
2016-07-06
Abstract
Copepods, small aquatic crustaceans, are the most abundant metazoan zooplankton and outnumber every other group of multicellular animals on earth. In spite of ecological and biological importance in aquatic environment, their morphological plasticity, originated from their various lifestyles and their incomparable capacity to adapt to a variety of environments, has made the identification of species challenging, even for expert taxonomists. Molecular approaches to species identification have allowed rapid detection, discrimination, and identification of cryptic or sibling species based on DNA sequence data. We examined sequence variation of a partial mitochondrial cytochrome C oxidase I gene (COI) from 133 copepod individuals collected from the Korean Peninsula, in order to identify and discriminate 94 copepod species covering six copepod orders of Calanoida, Cyclopoida, Harpacticoida, Monstrilloida, Poecilostomatoida and Siphonostomatoida. The results showed that there exists a clear gap with ca. 20 fold difference between the averages of within-specific sequence divergence (2.42%) and that of between-specific sequence divergence (42.79%) in COI, suggesting the plausible utility of this gene in delimitating copepod species. The results showed, with the COI barcoding data among 94 copepod species, that a copepod species could be distinguished from the others very clearly, only with four exceptions as followings: Mesocyclops dissimilis-Mesocyclops pehpeiensis (0.26% K2P distance in percent) and Oithona davisae-Oithona similis (1.1%) in Cyclopoida, Ostrincola japonica-Pseudomyicola spinosus (1.5%) in Poecilostomatoida, and Hatschekia japonica-Caligus quadratus (5.2%) in Siphonostomatoida. Thus, it strongly indicated that COI may be a useful tool in identifying various copepod species and make an initial progress toward the construction of a comprehensive DNA barcode database for copepods inhabiting the Korean Peninsula.
ISSN
1932-6203
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40382
DOI
10.1371/journal.pone.0157307
Bibliographic Citation
PLOS ONE, v.11, no.7, 2016
Publisher
PUBLIC LIBRARY SCIENCE
Subject
COMPLETE MITOCHONDRIAL GENOME; MOLECULAR PHYLOGENY; CALANOID COPEPODS; ATLANTIC COAST; COMPARATIVE PHYLOGEOGRAPHY; SPECIES IDENTIFICATION; TIGRIOPUS-CALIFORNICUS; CRUSTACEA-COPEPODA; ESTUARINE COPEPOD; ACARTIA-TONSA
Type
Article
Language
English
Document Type
Article
Publisher
PUBLIC LIBRARY SCIENCE
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