Genus-specific PCR Primers Targeting Intracellular Parasite Euduboscquella (Dinoflagellata: Syndinea) SCIE SCOPUS KCI

Cited 1 time in WEB OF SCIENCE Cited 1 time in Scopus
Title
Genus-specific PCR Primers Targeting Intracellular Parasite Euduboscquella (Dinoflagellata: Syndinea)
Author(s)
Jung, Jae-Ho; Choi, Jung Min; Kim, Young-Ok
KIOST Author(s)
Choi, Jung Min(최정민)Kim, Young Ok(김영옥)
Alternative Author(s)
최정민; 김영옥
Publication Year
2018-03
Abstract
We designed a genus-specific primer pair targeting the intracellular parasite Euduboscquella. To increase target specificity and inhibit untargeted PCR, two nucleotides were added at the 3' end of the reverse primer, one being a complementary nucleotide to the Euduboscquella-specific SNP (single-nucleotide polymorphism) and the other a deliberately mismatched nucleotide. Target specificity of the primer set was verified experimentally using PCR of two Euduboscquella species (positive controls) and 15 related species (negative controls composed of ciliates, diatoms and dinoflagellates), and analytical comparison with SILVA SSU rRNA gene database (release 119) in silico. In addition, we applied the Euduboscquella-specific primer set to four environmental samples previously determined by cytological staining to be either positive or negative for Euduboscquella. As expected, only positive controls and environmental samples known to contain Euduboscquella were successfully amplified by the primer set. An inferred SSU rRNA gene phylogeny placed environmental samples containing aloricate ciliates infected by Euduboscquella in a cluster discrete from Euduboscquella groups a-d previously reported from loricate, tintinnid ciliates.
ISSN
1738-5261
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40344
DOI
10.1007/s12601-017-0063-y
Bibliographic Citation
OCEAN SCIENCE JOURNAL, v.53, no.1, pp.81 - 90, 2018
Publisher
KOREA OCEAN RESEARCH DEVELOPMENT INST
Subject
MAXIMUM-LIKELIHOOD; DIVERSITY; REGIONS; MODELS
Keywords
marine parasite; syndinean dinoflagellate; SNP; target-specific PCR primer; ciliates
Type
Article
Language
English
Document Type
Article
Publisher
KOREA OCEAN RESEARCH DEVELOPMENT INST
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