Next-generation sequencing reveals the diversity of benthic diatoms in tidal flats
SCIE
SCOPUS
KCI
Cited 16 time in
WEB OF SCIENCE
Cited 19 time in
Scopus
-
Title
- Next-generation sequencing reveals the diversity of benthic diatoms in tidal flats
-
Author(s)
- An, Sung Min; Choi, Dong Han; Lee, Howon; Lee, Jung Ho; Noh, Jae Hoon
- KIOST Author(s)
- Choi, Dong Han(최동한); Lee, Ho Won(이호원)
-
Alternative Author(s)
- 안성민; 최동한; 이호원; 노재훈
-
Publication Year
- 2018-06
-
Abstract
- Benthic diatoms are ubiquitous in tidal flats and play major roles in maintaining coastal ecosystems. Spatio-temporal variations in diatom diversity have not been well-studied, mainly because of difficulties in morphological identification and the lack of appropriate genetic tools. To overcome these problems, we used the gene encoding the ribulose bisphosphate carboxylase large-subunit (rbcL) as a molecular marker, and sequenced these genes with the aid of the MiSeq platform. In this manner, we explored the genetic diversity of benthic diatoms in tidal flats of Guenso Bay on the west coast of Korea; differences in the spatial distributions of benthic diatoms were evident. The diatom communities were dominated by Nitzschia, Navicula, and Amphora; their relative distributions were affected by the sand proportion, grain size, and air exposure time. Our results suggest that meta-barcoding of the rbcL gene and next-generation sequencing can be used to explore the diversity of benthic diatoms.
-
ISSN
- 1226-2617
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/40338
-
DOI
- 10.4490/algae.2018.33.4.3
-
Bibliographic Citation
- ALGAE, v.33, no.2, pp.167 - 180, 2018
-
Publisher
- KOREAN SOC PHYCOLOGY
-
Subject
- SPECIES IDENTIFICATION; PHYLOGENETIC ANALYSIS; RDNA SEQUENCES; BACILLARIOPHYTA; COMMUNITIES; SEDIMENTS; GENE; RBCL; BARCODE; ESTUARY
-
Keywords
- benthic diatom; diversity; MiSeq; rbcL gene; tidal flat
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.