Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement
SCIE
SCOPUS
Cited 15 time in
WEB OF SCIENCE
Cited 15 time in
Scopus
-
Title
- Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement
-
Author(s)
- Jeong, Younsik; Choi, Yong-Woon; Park, Areumi; Lee, Yeon-Ji; Lee, Youngdeuk; Park, Gun-Hoo; Lee, Su-Jin; Lee, Won Kyu; Ryu, Yong Kyun; Kang, Do-Hyung
- KIOST Author(s)
- Choi, Yong Woon(최운용); Park, Areumi(박아름이); Lee, Yeon-Ji(이연지); Lee, Young Deuk(이영득); Park, Gun-Hoo(박건후); Lee, Won-Kyu(이원규); Ryu, Yong Kyun(유용균); Kang, Do Hyung(강도형)
-
Alternative Author(s)
- 정윤식; 최운용; 박아름이; 이연지; 이영득; 박건후; 이원규; 유용균; 강도형
-
Publication Year
- 2021-03
-
Abstract
- Serum is a stable medium supplement for in vitro cell culture. Live cells are used in stem cell research, drug toxicity and safety testing, disease diagnosis and prevention, and development of antibiotics, drugs, and vaccines. However, use of serum in culture involves concerns such as an ethical debate regarding the collection process, lack of standardized ingredients, and high cost. Herein, therefore, we evaluated the possibility of using edible cyanobacterium (Spirulina maxima), which is a nutrient-rich, sustainable, and ethically acceptable source, as a novel substitute for fetal bovine serum (FBS). H460 cells were cultured to the 10th generation by adding a mixture of spirulina animal cell culture solution (SACCS) and FBS to the culture medium. Cell morphology and viability, cell cycle, apoptosis, proteomes, and transcriptomes were assessed. We observed that SACCS had better growth-promoting capabilities than FBS. Cell proliferation was promoted even when FBS was replaced by 50-70% SACCS; there was no significant difference in cell shape or viability. There were only slight differences in the cell cycle, apoptosis, proteomes, and transcriptomes of the cells grown in presence of SACCS. Therefore, SACCS has the potential to be an effective, low-cost, and eco-friendly alternative to FBS in in vitro culture.
-
ISSN
- 2045-2322
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/40172
-
DOI
- 10.1038/s41598-021-84558-2
-
Bibliographic Citation
- SCIENTIFIC REPORTS, v.11, no.1, 2021
-
Publisher
- NATURE RESEARCH
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.