First molluscan transcription factor activator protein-1 (Ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury
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Title
- First molluscan transcription factor activator protein-1 (Ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury
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Author(s)
- De Zoysa, Mahanama; Nikapitiya, Chamilani; Lee, Youngdeuk; Lee, Sukkyoung; Oh, Chulhong; Whang, Ilson; Yeo, Sang-Yeop; Choi, Cheol Young; Lee, Jehee
- KIOST Author(s)
- Oh, Chul Hong(오철홍)
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Alternative Author(s)
- 오철홍
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Publication Year
- 2010-12
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Abstract
- The regulation of transcriptional activation is an essential and critical point in gene expression. In this study, we describe a novel transcription factor activator protein-1 (Ap-1) gene from disk abalone Haliotis discus discus (AbAp-1) for the first time in mollusk. It was identified by homology screening of an abalone normalized cDNA library. The cloned AbAp-1 consists of 945 bp coding region that encodes a putative protein containing 315 amino acids. The AbAp-1 gene is composed of a characteristic Jun transcription factor domain and a highly conserved basic leucine zipper (bZIP) signature similar to known Ap-1 genes. The AbAp-1 shares 46, 43 and, 40% amino acid identities with fish (Takifugu rubripes), human and insect (Ixodes scapularis) Ap-1, respectively. Quantitative real time RT-PCR analysis confirmed that AbAp-1 gene expression is constitutive in all selected tissues. AbAp-1 was upregulated in gills after bacteria (Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes) challenge; and, upregulated in hemocytes and gills by viral hemorrhagic septicemia virus (VHSV) challenge. Shell damage and tissue injury also increased the transcriptional level of Ap-1 in mantle together with other transcription factors (NF-kB, LITAF) and proinflammatory TNF-alpha. All results considered, identification and gene expression data demonstrate that abalone Ap-1 is an important regulator in innate immune response against bacteria and virus, as well as in the inflammatory response during tissue injury. In addition, stimulation of Ap-1 under different external stimuli could be useful to understand the Ap-1 biology and its downstream target genes, especially in abalone-like mollusks. (C) 2010 Elsevier Ltd. All rights reserved.
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ISSN
- 1050-4648
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4007
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DOI
- 10.1016/j.fsi.2010.08.014
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Bibliographic Citation
- FISH & SHELLFISH IMMUNOLOGY, v.29, no.6, pp.1028 - 1036, 2010
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Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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Subject
- NF-KAPPA-B; OXIDATIVE STRESS; GENE-EXPRESSION; MOLECULAR CHARACTERIZATION; CELL-PROLIFERATION; CLONING; JUN; HOMOLOG; FOS; MECHANISM
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Keywords
- Abalone; Haliotis discus discus; Invertebrate; Mollusk; Transcription factor activator protein-1(AP-1)
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Type
- Article
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Language
- English
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Document Type
- Article
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