선박평형수처리장치 성능 평가를 위한 해양 바이러스 생사판별 방법 개발 KCI

Title
선박평형수처리장치 성능 평가를 위한 해양 바이러스 생사판별 방법 개발
Alternative Title
Development of Marine Virus-like Particles Live/Dead Determination Method for the Performance Evaluation of Ballast Water Treatment System
Author(s)
Hyun, Bonggil; Woo, Joo Eun; Jang, Pung Guk; Jang, Min Chul; Lee, Woo Jin; Bae, Mi Kyung; Shin, Kyoungsoon
KIOST Author(s)
Hyun, Bonggil(현봉길)Woo, Joo Eun(우주은)Jang, Pung Guk(장풍국)Bae, Mi Kyung(배미경)Shin, Kyoung Soon(신경순)
Alternative Author(s)
현봉길; 우주은; 장풍국; 장민철; 이우진; 배미경; 신경순
Publication Year
2021-01
Abstract
본 연구는 향후 보다 강화될 것으로 예측되어지는 USCG Phase II 형식승인시험을 대비하기 위해 SYBR Green I과 SYBR Gold의 염색 효율을 비교한 후 염색 효율이 높은 시약을 실제 선박평형수처리장치(electrolysis type, UV + electrolysis type)를 통과한 처리수에 적용해서 보았다. 시료의 부피가 0.5 mL ∼ 2 mL, 염색 시약(Stock solution)을 100배 및 200배 희석한 조건에서 염색된 바이러스가 가장 선명하게 관찰되었다. SYBR Green I과 SYBR Gold의 염색효율은 장목한 해수조건의 실험구에서 유의한 차이를 보이지 않았지만, SYBR Gold의 노란색에 비해 SYBR Green I으로 염색된 시료에서 발현하는 녹색 형광이 보다 선명해서 관찰이 용이한 것으로 확인 되었다. 선박평형수처리장치(electrolysis type, UV + electrolysis type)를 통과하지 않은 실험수 및 대조수에서의 해양 바이러스 현존량은 약 109~1010 VLP 100 mL-1 으로 확인된 반면, 처리수에서는 살아 있는 바이러스가 관찰되지 않았다. 실험수 결과를 보면, SYBR Green I은 해수, 기수, 담수 조건에서 효과적으로 염색이 되는 것으로 확인되었다. 다양한 선박평형수처리기술에 따른 추가적인 검증 및 염색 방법 개발이 필요하지만, SYBR Green I 염색법은 USCG Phase II 미국형식승인시험 바이러스 생산판별에 좋은 대안이 될 수 있을 것으로 판단된다.


To prepare more stringent regulations for USCG Phase II ballast water management, this study investigated the staining efficiency of SYBR Green I(SGI) and SYBR Gold(SG) on the virus-like particle (VLP). A dye with high staining efficiency was applied to the treated water that was passed through the ballast water treatment system (BWTS). VLP staining was observed most clearly under the 100-fold and 200-fold dilution of the stock solution when the volume of filtered samples was 0.5 mL to 2 mL. The staining efficiency of SGI and SG did not show a significant difference. On the other hand, the green fluorescence of viruses in the sample stained with SGI was more pronounced than in the samples stained with SG (expressed yellow fluorescence), making it easier to observe. The abundance of VLP in the test water and control water treatments that did not pass through the two types of BWTS (electrolysis type, UV + electrolysis type) was approximately 109 - 1010 VLP 100 mL-1. In contrast, no stained VLP was observed in the treated water treatments. Moreover, SGI was confirmed to be effectively stained under various salinity conditions, including seawater, brackish water, and freshwater. Further verification tests and development of staining methods under various BWTS are required, but the SGI staining method is believed to be a good alternative to the VLP live/dead determination of the USCG Phase II type approval test.
ISSN
1975-4701
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40022
DOI
10.5762/KAIS.2021.22.1.431
Bibliographic Citation
한국산학기술학회논문지, v.22, no.1, pp.431 - 438, 2021
Publisher
한국산학기술학회
Keywords
USCG Phase II; BWTS; Virus-like particle; SYBR Green I; SYBR Gold
Type
Article
Language
Korean
Publisher
한국산학기술학회
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