In vivo comet assay on flounder and clam exposed to BaP and TBT SCOPUS KCI

Title
In vivo comet assay on flounder and clam exposed to BaP and TBT
Author(s)
Kim, S.J.; Chung, Y.; Lee, T.-K.
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2011
Abstract
The comet assay, also called single-cell electrophoresis (SCGE) assay, is a potential sensitive monitoring tool for DNA damage in cells. The primary objective of this study was to use comet assay to ascertain if the blood cells of flounder (Pleuronichthys olivaceus) and muscle cells of clam (Saxidomus purpurata) are suitable for genotoxicity screening. This was achieved by initially exposing blood and muscle cells under in vitro conditions to the reference genotoxin hydrogen peroxide (H 2O 2); strong correlation between H 2O 2 concentration and comet values were found. Subsequently, the identification of DNA damage in isolated cells from flounder and clam was performed under in vivo exposure to benzo(a)pyrene (BaP) and tributyltin (TBT). Flounder and clam were exposed to different concentrations (1, 10, 50, 100 μg/L) of BaP or TBT for 4 days. Regardless of treated chemicals, blood cells of flounder were more prone to DNA breakage compared to muscle cells of clam. In conclusion, in vivo genotoxicity of BaP and TBT can be biomonitored using the comet assay. This study suggests that flounder and clam do show potential as mediums for monitoring genotoxic damage by comet assay.
ISSN
1598-141X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3929
DOI
10.4217/OPR.2011.33.2.127
Bibliographic Citation
Ocean and Polar Research, v.33, no.2, pp.127 - 133, 2011
Subject
bioassay; biomonitoring; DNA; fish; genotoxicity; mollusc; organic pollutant; pollution exposure; tributyltin; Bivalvia; Pleuronectoidei; Pleuronichthys; Saxidomus purpurata
Keywords
BaP; Clam; Comet assay; Flounder; TBT
Type
Article
Language
English
Document Type
Article
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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