Bio-indigo production in two different fermentation systems using recombinant Escherichia coli cells harboring a flavin-containing monooxygenase gene (fmo) SCIE SCOPUS

Cited 23 time in WEB OF SCIENCE Cited 30 time in Scopus
Title
Bio-indigo production in two different fermentation systems using recombinant Escherichia coli cells harboring a flavin-containing monooxygenase gene (fmo)
Author(s)
Han, Gui Hwan; Bang, Seong Eun; Babu, Bandamaravuri Kishore; Chang, Man; Shin, Hyun-Jae; Kim, Si Wouk
Publication Year
2011-03
Abstract
Bio-indigo was produced in 3000-L batch and 5-L continuous fermentation of a recombinant Escherichia coli DH5 alpha harboring a novel gene encoding flavin-containing monooxygenase (FMO). Batch fermentation in a 3000-L fermenter produced 911 +/- 22 mg L-1 of indigo from 2 g L-1 tryptophan as a substrate (yield 46.9%) under the following culture conditions as follows: culture temperature 30 degrees C, pH 7.0, agitation speed 200 rpm, and aeration 3 vvm. Sufficient oxygen (aeration rate and agitation speed) was critical for bio-indigo production. For continuous fermentation in a 5-L fermenter, the volumetric productivity was found to be 11.3 mg L-1 h(-1) up to 110 h (final accumulated bio-indigo was 23 g) with a constant dilution rate (D) of 0.084 h(-1) (constant feeding rate of 0.167 L h(-1) with medium containing 3 g L-I tryptophan). Recombinant E. coli cells have the ability to withstand the toxicity of high concentration of accumulated indigo in batch fermentation. In continuous fermentation, the recombinant cells exhibited high plasmid stability up to 110 h, after which they lost the plasmid. (C) 2010 Elsevier Ltd. All rights reserved.
ISSN
1359-5113
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3909
DOI
10.1016/j.procbio.2010.10.015
Bibliographic Citation
PROCESS BIOCHEMISTRY, v.46, no.3, pp.788 - 791, 2011
Publisher
ELSEVIER SCI LTD
Subject
INDOLE; BIOSYNTHESIS; OXIDATION; ACID
Keywords
Bio-indigo; Plasmid stability; Flavin-containing monooxygenase; Batch fermentation; Continuous fermentation
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
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