RNA-Seq Transcriptome Analysis of the Cutlass Fish Reveals Photoreceptors Gene Expression in Peripheral Tissues
SCOPUS
KCI
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Title
- RNA-Seq Transcriptome Analysis of the Cutlass Fish Reveals Photoreceptors Gene Expression in Peripheral Tissues
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Author(s)
- Hyeon, Ji Yeon; Kim, Mun-Kwan; Lim, Bong-Soo; Byun, Jun Hwan; Moon, Ji-Sung; Kang, Hyeong-Cheol; Hur, Sung Pyo; Oh, Seong-Rip
- KIOST Author(s)
- Hyeon, Ji Yeon(현지연)
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Alternative Author(s)
- 현지연; 변준환; 허성표
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Publication Year
- 2017-06
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Abstract
- The opsin family of light sensitive proteins family makes up are the universal photoreceptor molecules of all visual systems in the vertebrates including teleosts. They can change their conformation from a resting state to a signaling state upon light absorption, which activates the G-protein coupled receptor, thereby resulting in a signaling cascade that produces physiological responses. However, this species is poorly characterized at molecular level due to little sequence information available in public databases. We have investigated the opsin family of nocturnal cutlass fish using the whole transcriptome sequencing method. The opsin genes were cloned and its expression in the tissues and organs were examined by qPCR. We cloned 6 opsin genes (RRH, Opn4, Rh1, Rh2, VA-opsin, and Opn3) in retina and brain tissue. It contained the seven presumed transmembrane domains that are characteristic of the G-protein-coupled receptor family. However, short wavelength sensitive pigment (SWS) and long wavelength sensitive pigment (LWS) were not detected in this study. The mRNA expression of the 6 photoreceptor genes were detected in retina and peripheral tissue. Our studies will lead to further investigation of the photic entrainment mechanism at molecular and cellular levels in cutlass fish and can be used in comparative studies of other fishes.
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ISSN
- 1598-141X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/38956
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DOI
- 10.4217/opr.2017.39.2.149
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Bibliographic Citation
- Ocean and Polar Research, v.39, no.2, pp.149 - 158, 2017
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Publisher
- 한국해양과학기술원
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Keywords
- cutlass fish; photoreceptors; opsin; transcriptome
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Type
- Article
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Language
- Korean
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