Dual activity of PNGM-1 pinpoints the evolutionary origin of subclass B3 metallo-beta-lactamases: a molecular and evolutionary study SCIE SCOPUS
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jung Hun | - |
dc.contributor.author | Takahashi, Masayuki | - |
dc.contributor.author | Jeon, Jeong Ho | - |
dc.contributor.author | Kang, Lin-Woo | - |
dc.contributor.author | Seki, Mineaki | - |
dc.contributor.author | Park, Kwang Seung | - |
dc.contributor.author | Hong, Myoung-Ki | - |
dc.contributor.author | Park, Yoon Sik | - |
dc.contributor.author | Kim, Tae Yeong | - |
dc.contributor.author | Karim, Asad Mustafa | - |
dc.contributor.author | Lee, Jung-Hyun | - |
dc.contributor.author | Nashimoto, Masayuki | - |
dc.contributor.author | Lee, Sang Hee | - |
dc.date.accessioned | 2020-12-10T08:00:52Z | - |
dc.date.available | 2020-12-10T08:00:52Z | - |
dc.date.created | 2020-05-27 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 2222-1751 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/38875 | - |
dc.description.abstract | Resistance to beta-lactams is one of the most serious problems associated with Gram-negative infections. beta-Lactamases are able to hydrolyze beta-lactams such as cephalosporins and/or carbapenems. Evolutionary origin of metallo-beta-lactamases (MBLs), conferring critical antibiotic resistance threats, remains unknown. We discovered PNGM-1, the novel subclass B3 MBL, in deep-sea sediments that predate the antibiotic era. Here, our phylogenetic analysis suggests that PNGM-1 yields insights into the evolutionary origin of subclass B3 MBLs. We reveal the structural similarities between tRNase Zs and PNGM-1, and demonstrate that PNGM-1 has both MBL and tRNase Z activities, suggesting that PNGM-1 is thought to have evolved from a tRNase Z. We also show kinetic and structural comparisons between PNGM-1 and other proteins including subclass B3 MBLs and tRNase Zs. These comparisons revealed that the B3 MBL activity of PNGM-1 is a promiscuous activity and subclass B3 MBLs are thought to have evolved through PNGM-1 activity. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Dual activity of PNGM-1 pinpoints the evolutionary origin of subclass B3 metallo-beta-lactamases: a molecular and evolutionary study | - |
dc.type | Article | - |
dc.citation.endPage | 1700 | - |
dc.citation.startPage | 1688 | - |
dc.citation.title | Emerging Microbes and Infections | - |
dc.citation.volume | 8 | - |
dc.citation.number | 1 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | Emerging Microbes and Infections, v.8, no.1, pp.1688 - 1700 | - |
dc.identifier.doi | 10.1080/22221751.2019.1692638 | - |
dc.identifier.scopusid | 2-s2.0-85075399344 | - |
dc.identifier.wosid | 000498718400001 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | STANDARD NUMBERING SCHEME | - |
dc.subject.keywordPlus | ANTIBIOTIC-RESISTANCE | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordAuthor | Antimicrobial resistance | - |
dc.subject.keywordAuthor | subclass B3 metallo-beta-lactamase | - |
dc.subject.keywordAuthor | tRNase Z | - |
dc.subject.keywordAuthor | dual activity | - |
dc.subject.keywordAuthor | structure and evolutionary origin | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Microbiology | - |