Graded expression of zinc-responsive genes through two regulatory zinc-binding sites in Zur SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Shin, Jung-Ho | - |
dc.contributor.author | Jung, Hoi Jong | - |
dc.contributor.author | An, Young Jun | - |
dc.contributor.author | Cho, Yoo-Bok | - |
dc.contributor.author | Cha, Sun-Shin | - |
dc.contributor.author | Roe, Jung-Hye | - |
dc.date.accessioned | 2020-04-20T07:44:01Z | - |
dc.date.available | 2020-04-20T07:44:01Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2011-03-22 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3882 | - |
dc.description.abstract | Zinc is one of the essential transition metals in cells. Excess or lack of zinc is detrimental, and cells exploit highly sensitive zinc-binding regulators to achieve homeostasis. In this article, we present a crystal structure of active Zur from Streptomyces coelicolor with three zinc-binding sites (C-, M-, and D-sites). Mutations of the three sites differentially affected sporulation and transcription of target genes, such that C-and M-site mutations inhibited sporulation and derepressed all target genes examined, whereas D-site mutations did not affect sporulation and derepressed only a sensitive gene. Biochemical and spectroscopic analyses of representative metal site mutants revealed that the C-site serves a structural role, whereas the M-and D-sites regulate DNA-binding activity as an on-off switch and a fine-tuner, respectively. Consistent with differential effect of mutations on target genes, zinc chelation by TPEN derepressed some genes (znuA, rpmF2) more sensitively than others (rpmG2, SCO7682) in vivo. Similar pattern of TPEN-sensitivity was observed for Zur-DNA complexes formed on different promoters in vitro. The sensitive promoters bound Zur with lower affinity than the less sensitive ones. EDTA-treated apo-Zur gained its DNA binding activity at different concentrations of added zinc for the two promoter groups, corresponding to free zinc concentrations of 4.5 x 10(-16) M and 7.9 x 10(-16) M for the less sensitive and sensitive promoters, respectively. The graded expression of target genes is a clever outcome of subtly modulating Zur-DNA binding affinities in response to zinc availability. It enables bacteria to detect metal depletion with improved sensitivity and optimize gene-expression pattern. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | FERRIC UPTAKE REGULATOR | - |
dc.subject | SHOCK SIGMA-FACTOR | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | BACILLUS-SUBTILIS | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject | STREPTOMYCES-COELICOLOR | - |
dc.subject | DEPENDENT DIMERIZATION | - |
dc.subject | RIBOSOMAL-PROTEINS | - |
dc.subject | HOMEOSTASIS | - |
dc.title | Graded expression of zinc-responsive genes through two regulatory zinc-binding sites in Zur | - |
dc.type | Article | - |
dc.citation.endPage | 5050 | - |
dc.citation.startPage | 5045 | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 108 | - |
dc.citation.number | 12 | - |
dc.contributor.alternativeName | 안영준 | - |
dc.contributor.alternativeName | 차선신 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.108, no.12, pp.5045 - 5050 | - |
dc.identifier.doi | 10.1073/pnas.1017744108 | - |
dc.identifier.scopusid | 2-s2.0-79953212617 | - |
dc.identifier.wosid | 000288712200067 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | FERRIC UPTAKE REGULATOR | - |
dc.subject.keywordPlus | SHOCK SIGMA-FACTOR | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject.keywordPlus | STREPTOMYCES-COELICOLOR | - |
dc.subject.keywordPlus | DEPENDENT DIMERIZATION | - |
dc.subject.keywordPlus | RIBOSOMAL-PROTEINS | - |
dc.subject.keywordPlus | HOMEOSTASIS | - |
dc.subject.keywordAuthor | ferric uptake regulator | - |
dc.subject.keywordAuthor | graded transcription regulation | - |
dc.subject.keywordAuthor | regulatory metal | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |