The mRNA expression patterns of kisspeptins, GnRHs, and gonadotropins in the brain and pituitary gland of a tropical damselfish, Chrysiptera cyanea, during the reproductive cycle
SCIE
SCOPUS
Cited 7 time in
WEB OF SCIENCE
Cited 7 time in
Scopus
-
Title
- The mRNA expression patterns of kisspeptins, GnRHs, and gonadotropins in the brain and pituitary gland of a tropical damselfish, Chrysiptera cyanea, during the reproductive cycle
-
Author(s)
- Imamura, Satoshi; Hur, Sung-Pyo; Takeuchi, Yuki; Badruzzaman, Muhammad; Mahardini, Angka; Rizky, Dinda; Takemura, Akihiro
-
Alternative Author(s)
- 허성표
-
Publication Year
- 2020-02
-
Abstract
- The sapphire devil (Chrysiptera cyanea) is a tropical damselfish that undergoes active reproduction under long-day conditions. To elucidate the physiological regulation of the brain-pituitary-gonadal axis in female sapphire devil, we cloned and characterized the genes of two kisspeptins (kiss1 and kiss2), three gonadotropin-releasing hormones (gnrh1, gnrh2, gnrh3), and the beta-subunit of two gonadotropins (fsh beta and lh beta) and investigated the gene expression changes during ovarian development. Quantitative polymerase chain reaction analyses in various brain parts revealed high expression levels of kiss1, kiss2, and gnrh2 in the diencephalon; gnrh2 and gnrh3 in the telencephalon; and fsh beta and lh beta in the pituitary. In situ hybridization (ISH) analyses revealed positive signals of kiss1 in the dorsal and ventral habenular nucleus and of kiss2 in the dorsal and ventral parts of the nucleus of the lateral recess. This analysis showed gnrh1 expression in the preoptic area (POA), suggesting that GnRH1 plays a stimulating role in the secretion of gonadotropins from the pituitary of the sapphire devil. High transcription levels of kiss1, kiss2, gnrh1, gnrh2, fsh beta, and lh beta were observed in the brain during the late vitellogenic stage, suggesting their involvement in the physiological processes of vitellogenesis. Immersion of fish in estradiol-17 beta (E2)-containing seawater resulted in increased expression of kiss2 and gnrh1 in their brains. This study showed that kiss-expressing neurons in the diencephalon are influenced by E2, leading to upregulation of gnrh1 in the POA and of fsh beta and lh beta in the pituitary during vitellogenesis.
-
ISSN
- 0920-1742
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/38743
-
DOI
- 10.1007/s10695-019-00715-5
-
Bibliographic Citation
- FISH PHYSIOLOGY AND BIOCHEMISTRY, v.46, no.1, pp.277 - 291, 2020
-
Publisher
- SPRINGER
-
Subject
- EUROPEAN SEA BASS; SOLE SOLEA-SENEGALENSIS; FOLLICLE-STIMULATING-HORMONE; 3 FORMS; GONADAL DEVELOPMENT; MOLECULAR-CLONING; PERCIFORM FISH; KISS2 NEURONS; RECEPTOR; SYSTEM
-
Keywords
- Coral reef; Damselfish; Gonadotropin-releasing hormone; In situ hybridization; Kisspeptin; Real-time PCR; Estradiol; Vitellogenesis
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.