Structural Evidence for Antihypertensive Effect of an Antioxidant Peptide Purified from the Edible Marine Animal Styela clava
SCIE
SCOPUS
KCI
Cited 22 time in
WEB OF SCIENCE
Cited 22 time in
Scopus
-
Title
- Structural Evidence for Antihypertensive Effect of an Antioxidant Peptide Purified from the Edible Marine Animal Styela clava
-
Author(s)
- Kang, Nalae; Ko, Seok-Chun; Kim, Hyun-Soo; Yang, Hye-Won; Ahn, Ginnae; Lee, Seung-Cheol; Lee, Tae-Gee; Lee, Jung-Suck; Jeon, You-Jin
- KIOST Author(s)
- Kang, Na Lae(강나래)
-
Alternative Author(s)
- 강나래
-
Publication Year
- 2020-02
-
Abstract
- This study investigated the antihypertensive effects of an antioxidant peptide, Leu-Trp-His-Thr-His (LWHTH), purified from Styela clava peptic hydrolysate, to assess the bioactivity of the peptide and verify the value of S. clava as a health-promoting food. Also, the study presented structural evidence for the effects of LWHTH. The inhibitory effect of LWHTH on angiotensin I-converting enzyme (ACE) was assessed using enzyme reaction methods and the simulation methods in computational space. LWHTH inhibited ACE with an IC50 value of 16.42 +/- 0.45 mu M. The LWHTH structure was stable, and its ACE inhibitory effect was retained under simulated gastrointestinal conditions. In silico simulations revealed that LWHTH binds the active site of ACE, with residues LW making the ACE-LWHTH complex stable and residues HTH making the complex strong. Furthermore, LWHTH significantly reduced blood pressure in spontaneously hypertensive rats. These results demonstrate that LWHTH has the potential to be a healthy functional food with antihypertensive effects. Therefore, S. clava consumption may be beneficial for human health.
-
ISSN
- 1096-620X
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/38741
-
DOI
- 10.1089/jmf.2019.4415
-
Bibliographic Citation
- JOURNAL OF MEDICINAL FOOD, v.23, no.2, pp.132 - 138, 2020
-
Publisher
- MARY ANN LIEBERT, INC
-
Keywords
- angiotensin I-converting enzyme; antioxidant peptide; blood pressure; molecular docking; Styela clava
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.