Structural Evidence for Antihypertensive Effect of an Antioxidant Peptide Purified from the Edible Marine Animal Styela clava SCIE SCOPUS KCI

Cited 3 time in WEB OF SCIENCE Cited 2 time in Scopus
Title
Structural Evidence for Antihypertensive Effect of an Antioxidant Peptide Purified from the Edible Marine Animal Styela clava
Author(s)
Kang, Nalae; Ko, Seok-Chun; Kim, Hyun-Soo; Yang, Hye-Won; Ahn, Ginnae; Lee, Seung-Cheol; Lee, Tae-Gee; Lee, Jung-Suck; Jeon, You-Jin
KIOST Author(s)
Kang, Nalae(강나래)
Publication Year
2020-02
Abstract
This study investigated the antihypertensive effects of an antioxidant peptide, Leu-Trp-His-Thr-His (LWHTH), purified from Styela clava peptic hydrolysate, to assess the bioactivity of the peptide and verify the value of S. clava as a health-promoting food. Also, the study presented structural evidence for the effects of LWHTH. The inhibitory effect of LWHTH on angiotensin I-converting enzyme (ACE) was assessed using enzyme reaction methods and the simulation methods in computational space. LWHTH inhibited ACE with an IC50 value of 16.42 +/- 0.45 mu M. The LWHTH structure was stable, and its ACE inhibitory effect was retained under simulated gastrointestinal conditions. In silico simulations revealed that LWHTH binds the active site of ACE, with residues LW making the ACE-LWHTH complex stable and residues HTH making the complex strong. Furthermore, LWHTH significantly reduced blood pressure in spontaneously hypertensive rats. These results demonstrate that LWHTH has the potential to be a healthy functional food with antihypertensive effects. Therefore, S. clava consumption may be beneficial for human health.
ISSN
1096-620X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38741
DOI
10.1089/jmf.2019.4415
Bibliographic Citation
JOURNAL OF MEDICINAL FOOD, v.23, no.2, pp.132 - 138, 2020
Publisher
MARY ANN LIEBERT, INC
Keywords
angiotensin I-converting enzyme; antioxidant peptide; blood pressure; molecular docking; Styela clava
Type
Article
Language
English
Document Type
Article
Publisher
MARY ANN LIEBERT, INC
Related Researcher
Research Interests

Marine Biotechnology,Bioactivities,Funcational materials,해양생물공학,생리활성,기능성 소재

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