Protective Effects of Fucoidan Isolated from Celluclast-Assisted Extract of Undaria pinnatifida Sporophylls against AAPH-Induced Oxidative Stress In Vitro and In Vivo Zebrafish Model SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Oh J.-Y. | - |
dc.contributor.author | Kim E.-A. | - |
dc.contributor.author | Kang S.I. | - |
dc.contributor.author | Yang H.-W. | - |
dc.contributor.author | Ryu B. | - |
dc.contributor.author | Wang L. | - |
dc.contributor.author | Lee J.-S. | - |
dc.contributor.author | Jeon Y.-J. | - |
dc.date.accessioned | 2020-12-10T07:51:08Z | - |
dc.date.available | 2020-12-10T07:51:08Z | - |
dc.date.created | 2020-06-01 | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/38667 | - |
dc.description.abstract | Fucoidan is a fucose-enriched polysaccharide, obtained from brown algae, with demonstrated antioxidant properties. However, traditional extraction methods using water or chemical-based extraction methods have reduced yield and produced hazardous by-products. In this study, we isolated fucoidan at a high yield using enzyme-assisted extraction; the Celluclast enzyme assisted extract of Undaria pinnatifida sporophylls (FCUS). To examine the antioxidant properties of FCUS, oxidative stress was induced with 2,2 ' -azobis (2-methylpropionamidine) dihydrochloride (AAPH) in Vero cells and zebrafish model. FCUS was composed of 30.4% sulfate and 52.3% fucose. Pre-treatment of Vero cells with FCUS dose dependently inhibited AAPH-induced reactive oxygen species (ROS) production. Moreover, FCUS remarkably reduced cell death, ROS generation, and lipid peroxidation production in zebrafish larvae. Overall, these findings indicate that the sulfate-rich fucoidan of FCUS, obtained with an eco-friendly process, could be implemented as a beneficial antioxidant agent in the functional food industry. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.title | Protective Effects of Fucoidan Isolated from Celluclast-Assisted Extract of Undaria pinnatifida Sporophylls against AAPH-Induced Oxidative Stress In Vitro and In Vivo Zebrafish Model | - |
dc.type | Article | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 25 | - |
dc.citation.number | 10 | - |
dc.contributor.alternativeName | 김은아 | - |
dc.identifier.bibliographicCitation | MOLECULES, v.25, no.10 | - |
dc.identifier.doi | 10.3390/molecules25102361 | - |
dc.identifier.scopusid | 2-s2.0-85085158022 | - |
dc.identifier.wosid | 000539293400094 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | ANTIOXIDANT ACTIVITY | - |
dc.subject.keywordPlus | SULFATED POLYSACCHARIDES | - |
dc.subject.keywordPlus | ENZYMATIC EXTRACTS | - |
dc.subject.keywordPlus | ANTITUMOR-ACTIVITY | - |
dc.subject.keywordPlus | ECKLONIA-CAVA | - |
dc.subject.keywordPlus | FUCAN | - |
dc.subject.keywordAuthor | zebrafish | - |
dc.subject.keywordAuthor | antioxidant | - |
dc.subject.keywordAuthor | Undaria pinnatifida sporophylls | - |
dc.subject.keywordAuthor | enzyme-assisted extraction | - |
dc.subject.keywordAuthor | fucoidan | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |