Protective Effects of Fucoidan Isolated from Celluclast-Assisted Extract of Undaria pinnatifida Sporophylls against AAPH-Induced Oxidative Stress In Vitro and In Vivo Zebrafish Model
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Title
- Protective Effects of Fucoidan Isolated from Celluclast-Assisted Extract of Undaria pinnatifida Sporophylls against AAPH-Induced Oxidative Stress In Vitro and In Vivo Zebrafish Model
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Author(s)
- Oh J.-Y.; Kim E.-A.; Kang S.I.; Yang H.-W.; Ryu B.; Wang L.; Lee J.-S.; Jeon Y.-J.
- KIOST Author(s)
- Kim, Eun A(김은아)
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Alternative Author(s)
- 김은아
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Publication Year
- 2020-05
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Abstract
- Fucoidan is a fucose-enriched polysaccharide, obtained from brown algae, with demonstrated antioxidant properties. However, traditional extraction methods using water or chemical-based extraction methods have reduced yield and produced hazardous by-products. In this study, we isolated fucoidan at a high yield using enzyme-assisted extraction; the Celluclast enzyme assisted extract of Undaria pinnatifida sporophylls (FCUS). To examine the antioxidant properties of FCUS, oxidative stress was induced with 2,2 ' -azobis (2-methylpropionamidine) dihydrochloride (AAPH) in Vero cells and zebrafish model. FCUS was composed of 30.4% sulfate and 52.3% fucose. Pre-treatment of Vero cells with FCUS dose dependently inhibited AAPH-induced reactive oxygen species (ROS) production. Moreover, FCUS remarkably reduced cell death, ROS generation, and lipid peroxidation production in zebrafish larvae. Overall, these findings indicate that the sulfate-rich fucoidan of FCUS, obtained with an eco-friendly process, could be implemented as a beneficial antioxidant agent in the functional food industry.
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ISSN
- 1420-3049
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/38667
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DOI
- 10.3390/molecules25102361
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Bibliographic Citation
- MOLECULES, v.25, no.10, 2020
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Publisher
- MDPI
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Keywords
- zebrafish; antioxidant; Undaria pinnatifida sporophylls; enzyme-assisted extraction; fucoidan
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Type
- Article
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Language
- English
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Document Type
- Article
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