Protective Effects of Fucoidan Isolated from Celluclast-Assisted Extract of Undaria pinnatifida Sporophylls against AAPH-Induced Oxidative Stress In Vitro and In Vivo Zebrafish Model SCIE SCOPUS

Cited 14 time in WEB OF SCIENCE Cited 14 time in Scopus
Title
Protective Effects of Fucoidan Isolated from Celluclast-Assisted Extract of Undaria pinnatifida Sporophylls against AAPH-Induced Oxidative Stress In Vitro and In Vivo Zebrafish Model
Author(s)
Oh J.-Y.; Kim E.-A.; Kang S.I.; Yang H.-W.; Ryu B.; Wang L.; Lee J.-S.; Jeon Y.-J.
KIOST Author(s)
Kim, Eun A(김은아)
Publication Year
2020-05
Abstract
Fucoidan is a fucose-enriched polysaccharide, obtained from brown algae, with demonstrated antioxidant properties. However, traditional extraction methods using water or chemical-based extraction methods have reduced yield and produced hazardous by-products. In this study, we isolated fucoidan at a high yield using enzyme-assisted extraction; the Celluclast enzyme assisted extract of Undaria pinnatifida sporophylls (FCUS). To examine the antioxidant properties of FCUS, oxidative stress was induced with 2,2 ' -azobis (2-methylpropionamidine) dihydrochloride (AAPH) in Vero cells and zebrafish model. FCUS was composed of 30.4% sulfate and 52.3% fucose. Pre-treatment of Vero cells with FCUS dose dependently inhibited AAPH-induced reactive oxygen species (ROS) production. Moreover, FCUS remarkably reduced cell death, ROS generation, and lipid peroxidation production in zebrafish larvae. Overall, these findings indicate that the sulfate-rich fucoidan of FCUS, obtained with an eco-friendly process, could be implemented as a beneficial antioxidant agent in the functional food industry.
ISSN
1420-3049
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38667
DOI
10.3390/molecules25102361
Bibliographic Citation
MOLECULES, v.25, no.10, 2020
Publisher
MDPI
Keywords
zebrafish; antioxidant; Undaria pinnatifida sporophylls; enzyme-assisted extraction; fucoidan
Type
Article
Language
English
Document Type
Article
Publisher
MDPI
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