Functional characterization of a delta 6-desaturase gene from the black seabream (Acanthopagrus schlegeli) SCIE SCOPUS

Cited 26 time in WEB OF SCIENCE Cited 26 time in Scopus
Title
Functional characterization of a delta 6-desaturase gene from the black seabream (Acanthopagrus schlegeli)
Author(s)
Kim, Sun Hee; Kim, Jong Bum; Kim, So Yun; Roh, Kyung Hee; Kim, Hyun Uk; Lee, Kyeong-Ryeol; Jang, Yo Soon; Kwon, Mi; Park, Jong Sug
Publication Year
2011-06
Abstract
Delta 6-fatty acid desaturase (D6DES) is used in the synthesis of polyunsaturated fatty acids (PUFAs) from microorganisms to higher animals, including arachidonic acid (ARA) and eicosapentaenoic acid (EPA). A 1,338 bp full-length cDNA encoding D6DES was cloned from Acanthopagrus schlegeli (AsD6DES) through degenerate- and RACE-PCR methods. A recombinant vector expressing AsD6DES (pYES-AsD6DES) was subsequently constructed and transformed into Saccharomyces cerevisiae to test the enzymatic activity of AsD6DES towards the production of n-6 and n-3 fatty acids. The exogenously expressed AsD6DES produced gamma-linolenic acid (18: 3 n-6) and stearidonic acid (18:4n-3) at 26 and 36% from exogenous linoleic acid (18:2n-6) and alpha-linolenic acid (18:3n-3), respectively, indicating that it is essentially a delta 6-fatty acid desaturase.
ISSN
0141-5492
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3861
DOI
10.1007/s10529-011-0555-2
Bibliographic Citation
BIOTECHNOLOGY LETTERS, v.33, no.6, pp.1185 - 1193, 2011
Publisher
SPRINGER
Subject
POLYUNSATURATED FATTY-ACIDS; DELTA-6-DESATURASE-LIKE ENZYME; NUTRITIONAL REGULATION; BRASSICA-JUNCEA; SPARUS-AURATA; FISH; DESATURASE; METABOLISM; CLONING; TROUT
Keywords
Acanthopagrus schlegeli; Delta 6-desaturase; Polyunsaturated fatty acids; Saccharomyces cerevisiae
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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