Expression profiling of liver in Java medaka fish exposed to 17 beta-estradiol
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Title
- Expression profiling of liver in Java medaka fish exposed to 17 beta-estradiol
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Author(s)
- Woo, Seonock; Jeon, Hye-Young; Lee, Taek-Kyun; Kim, Seong-Ryul; Lee, Seung-hoon; Yum, Seungshic
- KIOST Author(s)
- Woo, Seon Ock(우선옥); Lee, Taek Kyun(이택견); Yum, Seung Shic(염승식)
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Alternative Author(s)
- 우선옥; 이택견; 김성렬; 염승식
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Publication Year
- 2011-09-30
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Abstract
- Java medaka (Oryzias javanicus) cDNA array was constructed and the microarray platform was used to compare the hepatic expression profiles of Java medaka fish exposed to 17 beta-estradiol with those of unexposed controls. Data analysis demonstrated that the expression profiles were strongly affected by 17 beta-estradiol exposure, with 655 genes up- or downregulated after 24 h, and 633 genes after 48 h. The differentially expressed genes were analyzed to determine the effects of 17 beta-estradiol exposure on the liver tissue and were classified into five functional categories: information storage and processing, cellular processes and signaling, metabolism, general function prediction only, and function unknown. Genes whose expression was upregulated more than 10-fold were predominantly associated with energy production/conversion and reproduction, and 30% of the genes whose expression was downregulated more than 10-fold were associated with carbohydrate transport and metabolism. The observed differences in the expression profiles of 7 genes (encoding apolipoprotein B, cytochrome P450 1A, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase 1b, vitellogenin, selenoprotein M, and transferrin) were confirmed by quantitative RT-PCR, and their transcriptional changes in the livers of Java medaka induced by exposure to 10, 100, and 1,000 mu g/L 17 beta-estradiol were investigated. These results should allow the development of biomarkers for the identification of 17 beta-estradiol contamination in the environment and provide molecular biological information on the effects of endocrine-disrupting chemical exposure on marine animals.
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ISSN
- 1738-642X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3813
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DOI
- 10.1007/s13273-011-0033-x
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Bibliographic Citation
- MOLECULAR & CELLULAR TOXICOLOGY, v.7, no.3, pp.271 - 281, 2011
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Publisher
- KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
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Subject
- AQUATIC ENVIRONMENT; MARINE MEDAKA; ENDOCRINE; ESTROGEN; RESPONSES; STRESS
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Keywords
- Oryzias javanicus; Microarray; 17 beta-estradiol; Gene expression; Gene ontology
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Type
- Article
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Language
- English
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Document Type
- Article
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