Susceptibility to oxidative stress and modulated expression of antioxidant genes in the copper-exposed polychaete Perinereis nuntia SCIE SCOPUS

Cited 24 time in WEB OF SCIENCE Cited 32 time in Scopus
Title
Susceptibility to oxidative stress and modulated expression of antioxidant genes in the copper-exposed polychaete Perinereis nuntia
Author(s)
Won, Eun-Ji; Rhee, Jae-Sung; Kim, Ryeo-Ok; Ra, Kongtae; Kim, Kyung-Tae; Shin, Kyung-Hoon; Lee, Jae-Seong
KIOST Author(s)
Ra, Kong Tae(나공태)Kim, Kyung Tae(김경태)
Publication Year
2012-03
Abstract
To identify and evaluate potentially useful biomarkers for oxidative stress as early warning indices in the polychaete, Perinereis nuntia, we exposed P. nuntia to copper (Cu) and measured several biomarker enzymes (glutathione S-transferase; GST, glutathione peroxidase; GPx, Metallothionein-like protein; MTLPs, and catalase; CAT) and genes (Pn-GSTs. Pn-CAT, and Pn-MT) with a cellular oxidative index, reactive oxygen species (ROS) level. Accumulated Cu concentrations in P. nuntia increased in a time-dependent manner. Intracellular ROS reached high levels 6 h after exposure in P. nuntia with an increase of GST activity and glutathione (GSH) content. Particularly, GSH in polychaetes showed a positive correlation with Cu contents accumulated in P. nuntia. Messenger RNA expressions of GST sigma and GST omega showed relatively high expressions at 50 mu g/L of Cu exposure, even though the moderate increase of rest of GST isoforms was also observed. Also regarding long-term exposure, we reared P. nuntia in sediments for 15 days, and found that there was an obvious increase of Pn-GSTs, Psi-CAT, and Pn-MT genes with elevated concentrations of Cu and Cd in polychaete body, compared to initial levels, suggesting that P. nuntia in sediment was affected by metals as well as by other organic pollutants to induce oxidative stress genes and enzymes. These findings suggest that oxidative stress is a potential modulator of defense system of P. nuntia. Several potential biomarker genes are available as early warning signals for environmental biomonitoring. (C) 2011 Elsevier Inc. All rights reserved.
ISSN
1532-0456
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3614
DOI
10.1016/j.cbpc.2011.10.002
Bibliographic Citation
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v.155, no.2, pp.344 - 351, 2012
Publisher
ELSEVIER SCIENCE INC
Subject
LAEONEREIS-ACUTA POLYCHAETA; GLUTATHIONE; METALLOTHIONEIN; TRANSFERASE; RESPONSES; BINDING; BIOMARKERS; INDUCTION; TOXICITY; CADMIUM
Keywords
Copper; Reactive oxygen species; Glutathione S-transferase; Catalase; Metallothionein; Perinereis nuntia
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE INC
Related Researcher
Research Interests

Management of marine environment,Marine pollution,Trace metal(Heavy metal),해양환경관리,해양오염,미량금속(중금속)

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