Toxaphene affects the levels of mRNA transcripts that encode antioxidant enzymes in Hydra SCIE SCOPUS

Cited 10 time in WEB OF SCIENCE Cited 9 time in Scopus
Title
Toxaphene affects the levels of mRNA transcripts that encode antioxidant enzymes in Hydra
Author(s)
Woo, Seonock; Lee, Aekyung; Won, Hyokyoung; Ryu, Jae-Chun; Yum, Seungshic
KIOST Author(s)
Lee, Na Yun(이나윤)Yum, Seung Shic(염승식)
Publication Year
2012-06
Abstract
We evaluated toxaphene-induced acute toxicity in Hydra magnipapillata. The median lethal concentrations of the animals (LC50) were determined to be 34.5 mg/L, 25.0 mg/L and 12.0 mg/L after exposure to toxaphene for 24 h, 48 h and 72 h, respectively. Morphological responses of hydra polyps to a range of toxaphene concentrations suggested that toxaphene negatively affects the nervous system of H. magnipapillata. We used real-time quantitative PCR of RNA extracted from polyps exposed to two concentrations of toxaphene (0.3 mg/L and 3 mg/L) for 24 h to evaluate the differential regulation of levels of transcripts that encode six antioxidant enzymes (CAT, G6PD, GPx, GR, GST and SOD), two proteins involved in detoxification and molecular stress responses (CYP1A and UB), and two proteins involved in neurotransmission and nerve cell differentiation (AChE and Hym-355). Of the genes involved in antioxidant responses, the most striking changes were observed for transcripts that encode GPx, G6PD, SOD, CAT and GST, with no evident change in levels of transcripts encoding GR. Levels of UB and CYP1A transcripts increased in a dose-dependent manner following exposure to toxaphene. Given that toxaphene-induced neurotoxicity was not reflected in the level of AChE transcripts and only slight accumulation of Hym-355 transcript was observed only at the higher of the two doses of toxaphene tested, there remains a need to identify transcriptional biomarkers for toxaphene-mediated neurotoxicity in H. magnipapillata. Transcripts that respond to toxaphene exposure could be valuable biomarkers for stress levels in H. magnipapillata and may be useful for monitoring the pollution of aquatic environments. (C) 2012 Elsevier Inc. All rights reserved.
ISSN
1532-0456
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3580
DOI
10.1016/j.cbpc.2012.03.005
Bibliographic Citation
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v.156, no.1, pp.37 - 41, 2012
Publisher
ELSEVIER SCIENCE INC
Subject
MEDAKA ORYZIAS-JAVANICUS; DEVELOPMENTAL MECHANISMS; GENETIC-ANALYSIS; TOXICITY; MAGNIPAPILLATA; DIFFERENTIATION; ORGANISMS; CADMIUM; METALS; CELLS
Keywords
Toxaphene; Hydra magnipapillata; Oxidative stress; Antioxidant enzyme genes; Differential gene expression
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE INC
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