Photo-oxidative stress by ultraviolet-B radiation and antioxidative defense of eckstolonol in human keratinocytes
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Title
- Photo-oxidative stress by ultraviolet-B radiation and antioxidative defense of eckstolonol in human keratinocytes
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Author(s)
- Jang, Jiyi; Ye, Bo-Ram; Heo, Soo-Jin; Oh, Chulhong; Kang, Do-Hyung; Kim, Ji Hyung; Affan, Abu; Yoon, Kon-Tak; Choi, Young-Ung; Park, Se Chang; Han, Seunghee; Qian, Zhong-Ji; Jung, Won-Kyo; Choi, Il-Whan
- KIOST Author(s)
- Heo, Soo Jin(허수진); Oh, Chul Hong(오철홍); Kang, Do Hyung(강도형); Choi, Young Ung(최영웅)
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Alternative Author(s)
- 장지이; 예보람; 허수진; 오철홍; 강도형; 김지형; Abu Affan; 윤건탁; 최영웅
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Publication Year
- 2012-11
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Abstract
- Ultraviolet-B (UV-B) irradiation has been known to generate oxidative stress by increasing reactive oxygen species (ROS) in skin cells. Several naturally occurring antioxidant compounds isolated from marine algae are believed to protect against ROS. In this study, we assessed the antioxidative effect of eckstolonol isolated from Ecklonia cava against UV-B-induced ROS in human keratinocytes (HaCaTs). We investigated the effects of photo-oxidative stress by UV-B (50 mJ/cm(2)) and the antioxidative effects of eckstolonol using fluorometry, flow cytometry, microscopy, and cell viability and comet assays. UV-B irradiation decreased cell viability, which was restored in a dose-dependent manner with eckstolonol treatment (0, 5, 50, 100, and 200 mu M). Moreover, eckstolonol reduced UV-B-induced ROS, lipid peroxidation, damaged DNA levels, and cell death. These antioxidative effects seem to be due to the enzymatic activities of catalase (CAT) and superoxide dismutase (SOD). Collectively, these results indicate that eckstolonol is capable of protecting keratinocytes from photo-oxidative stress. (C) 2012 Elsevier B.V. All rights reserved.
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ISSN
- 1382-6689
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3431
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DOI
- 10.1016/j.etap.2012.08.003
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Bibliographic Citation
- ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, v.34, no.3, pp.926 - 934, 2012
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Publisher
- ELSEVIER
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Subject
- MEDIATED DAMAGE; TRIPHLORETHOL-A; ECKLONIA-CAVA; IN-VIVO; OXYGEN; MELANOGENESIS; PHLOROTANNINS; ASSAY
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Keywords
- Antioxidant; Ecklonia cava; Eckstolonol; Human keratinocytes; Oxidative stress; Ultraviolet-B
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Type
- Article
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Language
- English
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Document Type
- Article
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