CO-Dependent H-2 Production by Genetically Engineered Thermococcus onnurineus NA1
SCIE
SCOPUS
Cited 66 time in
WEB OF SCIENCE
Cited 75 time in
Scopus
-
Title
- CO-Dependent H-2 Production by Genetically Engineered Thermococcus onnurineus NA1
-
Author(s)
- Kim, Min-Sik; Bae, Seung Seob; Kim, Yun Jae; Kim, Tae Wan; Lim, Jae Kyu; Lee, Seong Hyuk; Choi, Ae Ran; Jeon, Jeong Ho; Lee, Jung-Hyun; Lee, Hyun Sook; Kang, Sung Gyun
- KIOST Author(s)
- Kim, Yun Jae(김윤재); Lim, Jae Kyu(임재규); Lee, Seong Hyuk(이성혁); Lee, Jung Hyun(이정현); Lee, Hyun Sook(이현숙); Kang, Sung Gyun(강성균)
-
Alternative Author(s)
- 김민식; 배승섭; 김윤재; 김태완; 임재규; 이성혁; 최애란; 전정호; 이정현; 이현숙; 강성균
-
Publication Year
- 2013-03
-
Abstract
- Hydrogenogenic CO oxidation (CO + H2O -> CO2 + H-2) has the potential for H-2 production as a clean renewable fuel. Thermococcus onnurineus NA1, which grows on CO and produces H-2, has a unique gene cluster encoding the carbon monoxide dehydrogenase (CODH) and the hydrogenase. The gene cluster was identified as essential for carboxydotrophic hydrogenogenic metabolism by gene disruption and transcriptional analysis. To develop a strain producing high levels of H-2, the gene cluster was placed under the control of a strong promoter. The resulting mutant, MC01, showed 30-fold-higher transcription of the mRNA encoding CODH, hydrogenase, and Na+/H+ antiporter and a 1.8-fold-higher specific activity for CO-dependent H-2 production than did the wild-type strain. The H-2 production potential of the MC01 mutant in a bioreactor culture was 3.8-fold higher than that of the wild-type strain. The H-2 production rate of the engineered strain was severalfold higher than those of any other CO-dependent H-2-producing prokaryotes studied to date. The engineered strain also possessed high activity for the bioconversion of industrial waste gases created as a by-product during steel production. This work represents the first demonstration of H-2 production from steel mill waste gas using a carboxydotrophic hydrogenogenic microbe.
-
ISSN
- 0099-2240
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3262
-
DOI
- 10.1128/AEM.03298-12
-
Bibliographic Citation
- APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.79, no.6, pp.2048 - 2053, 2013
-
Publisher
- AMER SOC MICROBIOLOGY
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.