CO-Dependent H-2 Production by Genetically Engineered Thermococcus onnurineus NA1 SCIE SCOPUS

Cited 54 time in WEB OF SCIENCE Cited 58 time in Scopus
Title
CO-Dependent H-2 Production by Genetically Engineered Thermococcus onnurineus NA1
Author(s)
Kim, Min-Sik; Bae, Seung Seob; Kim, Yun Jae; Kim, Tae Wan; Lim, Jae Kyu; Lee, Seong Hyuk; Choi, Ae Ran; Jeon, Jeong Ho; Lee, Jung-Hyun; Lee, Hyun Sook; Kang, Sung Gyun
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2013-03
Abstract
Hydrogenogenic CO oxidation (CO + H2O -> CO2 + H-2) has the potential for H-2 production as a clean renewable fuel. Thermococcus onnurineus NA1, which grows on CO and produces H-2, has a unique gene cluster encoding the carbon monoxide dehydrogenase (CODH) and the hydrogenase. The gene cluster was identified as essential for carboxydotrophic hydrogenogenic metabolism by gene disruption and transcriptional analysis. To develop a strain producing high levels of H-2, the gene cluster was placed under the control of a strong promoter. The resulting mutant, MC01, showed 30-fold-higher transcription of the mRNA encoding CODH, hydrogenase, and Na+/H+ antiporter and a 1.8-fold-higher specific activity for CO-dependent H-2 production than did the wild-type strain. The H-2 production potential of the MC01 mutant in a bioreactor culture was 3.8-fold higher than that of the wild-type strain. The H-2 production rate of the engineered strain was severalfold higher than those of any other CO-dependent H-2-producing prokaryotes studied to date. The engineered strain also possessed high activity for the bioconversion of industrial waste gases created as a by-product during steel production. This work represents the first demonstration of H-2 production from steel mill waste gas using a carboxydotrophic hydrogenogenic microbe.
ISSN
0099-2240
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3262
DOI
10.1128/AEM.03298-12
Bibliographic Citation
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.79, no.6, pp.2048 - 2053, 2013
Publisher
AMER SOC MICROBIOLOGY
Type
Article
Language
English
Document Type
Article
Publisher
AMER SOC MICROBIOLOGY
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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