Bioluminescent Assay of Farnesyl Protein Transferase (FPTase) Using Bacterial Luciferase from Vibrio harveyi

Title
Bioluminescent Assay of Farnesyl Protein Transferase (FPTase) Using Bacterial Luciferase from Vibrio harveyi
Author(s)
조기웅; 박승일; 방지헌
Publication Year
2003-05-02
Abstract
Instead of the usual assay method of FPTase using radioisotope labelled farnesyl pyrophosphate and synthetic peptide (-CXXA-) as substrates, a luminous assay method using bacterial luciferase as substrate was designed. Luciferase from marine luminous bacterium V. harveyi has highly active Cys. residue in its active site and lose activity by any modifying agent such as 2-bromo-decanal. In the presence of FPTase, added farnesyl pyrophosphate acts as farnesylation agent to bacterial luciferase Cys-106 sulfhydryl group and inactivate the luciferase activity completely while other luciferase from V. fischeri without Cys-106 sulfhydryl group didn't show irreversible inhibition. This result can be developed to a new non-radioisotopic assay method of FPTase which is a very important target for anticancer drug development
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/32335
Bibliographic Citation
한국미생물학회춘계학술대회, pp.198, 2003
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse