Exopolysaccharide from Colorless Mutant of Hahella chejuensis strain 96CJ10356

Abstract

Red pigment forming Hahella chejuensis 96CJ10356 producde novel viscous exopolysaccharide(EPS), named as EPS-R. In order to prepare colorless EPS, mutants of H. chejuensis 96CJ10356 were developed using by EMS (ethylmetane sulfonate) mutagenesis. Strain m10356, one of non-pigment forming mutants, produced white colored EPS, p-m10356 with higher productivity. The characteristics of p-m10356 such as average molecular masses, sugar compositions, element composition, thermal properties were analyzed. After the effects of salt, pH, temperature, inorganic compounds, and C, N-source were tested, M-m10356 medium was proposed for optimal EPS production. Rheological properties of p-M10356 at various temperatures, concentrations, salts, pH and mixed other polysaccharide were monitored using oscillatory measurement and steady-shear viscosity measurement.