자가와 타가 영양방법으로배양되는 Dunaliella salina

Abstract

To obtain the economic benefits of biomass production, the maximum growth rate of Dunaliella was induced by use of the mixotrophic culture. Initially, heterotrophic capability of Dunaliella has been investigated with 3 different Dunaliella strains including D. bardawill, D. salina (UTEX 1644) and D. salina-155. D. salina-155 has been selected from mutated D. salina (UTEX 1644). Three different strains grow autotrophically with the same culture condition described in (Jin et al. 2003 in Biotech. and Bioengin.: 81:115-125). However, when the carbon source, sodium bicarbonate was replaced with sodium acetate, only D. salina-155 grew actively in acetate containing media and reached same final cell density as other strains grow photo-autotrophically. When D. salina-155 was subjected to mixotrophic growth condition, final cell density of this strain was higher by 50 % than that of control. This mixotrophic growth (acetate media or glucose media with light) of D. salina is discussed in terms of its potential utilization by the algal biotechnology industry for production of high-value bioproduct.