Culture & Phylogenetic Analysis of The Arctic Bacteria

Title
Culture & Phylogenetic Analysis of The Arctic Bacteria
Author(s)
이유경; 김효원; 강성호; 이홍금; 김예동
Alternative Author(s)
이유경; 김효원; 강성호; 이홍금; 김예동
Publication Year
2003-09-22
Abstract
We isolated the Arctic microorganisms, identified using 16S rDNA sequences, and preserved in deep-freezer as a genetic bank. We collected soil microorganisms that have inhabited around Korean Arctic Research Station Dasan located at Ny-Alsund, Svalbard, Norway. The collected soils were diluted in distilled water, the diluted water was spread on petri-films, and the petri-films were transferred to the laboratory at KORDI. The petri-films were cultured at 4 for 1 month; colonies were succeedingly cultured on nutrient agar plate 4 for every 7days. The pure colonies were inoculated into nutrient agar liquid media, and genomic DNA was extracted from the liquid-cultured strains. The total 221 viable microorganisms and 203 genomic DNAs were preserved in deep-freezer. Among the 221 strains, the 16S rDNAs of 97 strains were amplified, sequenced, and phylogenetically analyzed. Out of 97 strains, 21 strains were overlapped and 76 microorganisms were identified: 55 species of Pseudomonas genus, 21 species of Arthrobacter genus, Alcaligenes sp., Phychrobacter sp., and Yersinia sp. We expect that these Arctic microorganisms isolated in this study can be used for screening to develop new antibiotics or industrial enzymes.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/32193
Bibliographic Citation
Marine Biotechnology Conference, pp.134, 2003
Publisher
Marine Biotechnology Conference
Type
Conference
Language
English
Publisher
Marine Biotechnology Conference
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