단백질 분해 효소를 생산하는 북극 세균의 계통 분석

Abstract

The Arctic is a representative cold habitat that offers good sources of useful enzymes with activity at low temperature. These enzymes are stable after long time storing and some times freeze-thaw cycles. Among the enzymes, protease is important enzyme at medical industry, environment industry, food industry, chemical industry etc. We isolated soil and marine samples from around Korean Arctic Research Station Dasan located at Ny-Alsund, Svalbard, Norway (79°N, 12°E). The collected samples were diluted in distilled water and seawater, and spread on nutrient and marine agar plates. The samples and plates were transferred to the laboratory at KORDI. They cultured at 10℃, colonies were succeedingly cultured on nutrient and zobell agar plates at 10℃. The pure colonies were inoculated into nutrient and zobell liquid media, and genomic DNA were extracted from the liquid-cultured strains. Phylogenetic analysis using 16S rDNA indicated that the protease-producing bacteria belong to Achromobacter, Arthrobacter, Bacillus, Carnobacterium, Flavobacterium, Paracoccus, Pedobacter, Pseudoaltermonas, Pseudomonas, Psychrobacter, and Staphylococcus species. Many of them are candidates for new species and sources of cold-active protease. We expect that these Arctic bacteria can be used for screening to develop new industrial enzymes.