Hahella chejuensis 96CJ10356의 돌연변이체 유도

Title
Hahella chejuensis 96CJ10356의 돌연변이체 유도
Alternative Title
Mutagenesis of Hahella chejuensis 96CJ10356 using EMS & Characterization of the Exopolysaccharide Produced by a Mutant Strain
Author(s)
이유경; 임정한; 이미란; 조현희; Yin Ruochun; 이홍금
Alternative Author(s)
이유경; 임정한; 이미란; 조현희; 이홍금
Publication Year
2004-05-13
Abstract
We isolated 155 mutants of Hahella chejuensis 96CJ10356 by EMS(Ethylmethane Sulfonate) mutagenesis method. Hahella chejuensis is a novel marine bacterium that produces a large amount of novel exopolysaccharide (EPS) and red pigment. Hahella chejuensis was cultured in STN liquid medium at 25℃. For mutagenesis, 0.2 M of EMS was added into cell suspension and the mixture was incubated for 1 hr at 25℃. Na2S2O3 was added to stop the reaction of EMS at a final concentration of 6%. The cells were diluted and spread on plates; colonies showing different color or shape were selected. Total 155 mutant strains were preserved in glycerol media (15%, v/v) at –80℃. One of the white mutants, m10356, produced white colored EPS, p-m10356. The mutant m10356 showed higher productivity of exopolysaccharide than wild type, and the exopolysaccharide p-m10356 possessed good emulsify stability under broad range of temperature and pH conditions. Other mutants with diverse colors are going to use for screening of genes in charge of the pigment biosynthesis.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31936
Bibliographic Citation
International Meeting of the Microbiological Society of Korea, pp.197, 2004
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
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