북극 생물막 미생물의 분리

Abstract

The Arctic is a representative cold habitat that offers good sources of useful enzymes with activity at low temperature. We isolated biofilm from a floating pier around Korean Arctic Research Station Dasan located at Ny-Alsund, Svalbard, Norway (79°N, 12°E). The collected samples were diluted in distilled seawater, and spread on marine agar plates. The plates were transferred to the laboratory at KORDI under cold condition. They cultured at 10℃, and colonies were succeeding cultured on zobell agar plates at 10℃. The pure colonies were inoculated into zobell liquid media, and genomic DNA was extracted from the liquid-cultured strains. Phylogenetic analysis using 16S rDNA indicated that the biofilm bacteria belong to Glaciecola mesophila, marinobacter hydrocarbonoclasticus, Marinomonas protea, Planococcus southpolar, Polaribacter irgensii, Pseudoaltermonas agarovorans, Pseudoaltermonas citrea, Pseudomonas anguilliseptica, Psychrobacter fozii, and Shewanella frigidimarina. We expect that these Arctic bacteria can be used for screening to develop new industrial enzymes.