The formation of biofilm in marine environments is starting point of biofouling and/or biocorrosion. However there were not enough informations about the bacteria concerned in early phase of biofilm formation and its control in marine environments. To procure standard bacterial strains for biofilm studies we isolated bacteria from early phase of biofilm and characterizing their properties related with biofilm formation. Acrylic surfaces were emerged in near shore seawater for 3 days at five areas of Korean Peninsula and bacterial isolates were obtained using ZoBell 2216e agar medium. Based on the morphological characteristics, 76 strains were selected and partial sequences of 16S rDNA (300 to 500 bps) were analyzed. Attachment properties on polystyrene surface according to Loo et al. (2002) and cell surface hydrophobicity by BATH method were analyzed. The isolates could be assigned to 26 known genera which have been reported as widespread in marine environment. Approximately 35% of the isolates had been reported previously as uncultured or unidentified in GenBank database. Properties related to biofilm formation of each strain will be described.