Novosphingobium pemtaromativorans균주의 고분자 PAHs분해에서 HPCD의 유용성

Abstract

Novosphingobium pentaromativorans US6-1, isolated from muddy sediment of Ulsan Bay is able to degrade polycyclic aromatic hydrocarbons. The degradation rate of 5 kinds of PAHs (each 10ppm of pyrene, chrysene, benz[a]anthrathene, benz[b]fluoanthene and benzo[a]pyrene) by strain US6-1 was ranged between 5 and 38% within 7 days when supplemented as a sole sources of carbon and energy. On the other hand PAHs degradation rates were increased to 50~99% when supplied with 10% of 2-hydroxypropyl-β-cyclodextrin (β-HPCD) as an additive. However the individual addition of surfactants (Tween-80, Tritonx-100, Brij 35; each 10xCMC) and/or co-substrates (1%glucose and 1%dextrin) did not enhancing the degradation rate of benzo[a]pyrene except the combination of dextrin and tween-80, degradation rate of this combination was 60%. These evidences imply that degradation of high molecular weight PAHs by strain US6-1 was progressed by cometabolism with appropriate carbon source and enhanced by addition of surfactant. Supplementation of β-HPCD with strain US6-1 was one of the most effective and promisable tools for the bioremediation of high molecular weight PAHs.