한국산 볼락의 유전적 다양성 분석

Title
한국산 볼락의 유전적 다양성 분석
Alternative Title
Genetic diversity of dark-banded rockfish (Sebastes inermis) in Korea revealed by RAPD and mitochondrial DNA analyses
Author(s)
홍경표; 장요순; 노충환; 명정구; 김종만
KIOST Author(s)
Noh, Choong Hwan(노충환)Myoung, Jung Goo(명정구)
Publication Year
2004-11-30
Abstract
Genetic diversity of dark-banded rockfish (Sebastes inermis) in wild and cultured stocks was examined by random amplified polymorphic DNA (RAPD) and mitochondrial DNA (control region) polymorphism analysis. Total of 46 polymorphic fragments were produced from 9 random primers, OPA-01, OPA-11, OPB-08, OPB-20, OPE-05, OPG-10, OPH-12, OPI-04 and OPI-20, with 7, 3, 6, 20, 6, 6, 10, 4 and 5 genotypes, respectively. Among OPB-08 depended polymorphic bands, 300 bp fragment appeared only in wild population at 61.3% frequency level, and suggested that this RAPD would be a useful marker for the discrimination of fish stock, wild or cultured, due to an effective parent number of each stock. In restriction fragment length polymorphism (PCR-RFLP) analysis of mtDNA control region with Mbo I and Xsp I, 8 mtDNA composite haplotypes were observed with different haplotype frequency and stock-specific haplotypes. It is expected that RAPD marker and mtDNA haplotypes identified in this study would be used as effective genetic monitoring tools in our ongoing stock enhancement program of Korea.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31607
Bibliographic Citation
7th Asian Fisheries Form, pp.10 - 11, 2004
Publisher
아시아수산학회
Type
Conference
Language
English
Publisher
아시아수산학회
Related Researcher
Research Interests

Fish genetics and breeding,Living modified organism,Sbtropical mariculture,어류유전육종,어류형질전환,아열대어류양식

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